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Maus IgG2a isotype control (FITC)

FACS, IsoC, IF IgG2a FITC
Produktnummer ABIN2145385
  • Target Alle IgG2a Produkte
    IgG2a
    Wirt
    • 185
    • 74
    • 3
    • 1
    Maus
    Konjugat
    FITC
    Applikation
    Flow Cytometry (FACS), Isotype Control (IsoC), Immunofluorescence (IF)
    Produktmerkmale
    This antibody can be used as a mouse IgG2a isotype control in flow cytometry and other applications.
    Isotyp
    IgG2a
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdurchführung
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 20 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    IgG2a
    Abstract
    IgG2a Produkte
    Synonyme
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    Substanzklasse
    Antibody
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