Ratte IgG isotype control (Alkaline Phosphatase (AP))
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- Target Alle IgG Produkte
- IgG
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Wirt
- Ratte
- Konjugat
- Alkaline Phosphatase (AP)
- Applikation
- Isotype Control (IsoC)
- Hersteller Produkt- Nr.
- 012-0502
- Hersteller
- Rockland
- Verwendungszweck
- Rat IgG Whole Molecule Alkaline Phosphatase Conjugated
- Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat Serum and anti-Alkaline Phosphatase (calf intestine).
- Produktmerkmale
- Rat IgG alkaline phosphatase conjugate, Rat IgG alk phos conjugate
- Aufreinigung
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by conjugation and extensive dialysis against the buffer stated above.
- Sterilität
- Sterile filtered
- Isotyp
- IgG
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- Applikationshinweise
- Optional[Flow Cytometry Dilution]: Rat IgG Alkaline Phosphatase Conjugated Whole Molecule Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
- Background: Rat IgG Alkaline Phosphatase Conjugated Whole Molecule - secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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