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Maus IgG2b Isotyp-Kontrolle

Name: Maus IgG2b Isotyp-Kontrolle
Brand: antibodies-online
SKU: ABIN1043029
Price: 311.8 EUR
Availability: InStock

FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG2b Isotype control (MPC-11) purified antibody (concentration in sample 2 μg/mL). (Maus IgG2b Isotyp-Kontrolle)

FACS

1 image

(5 Referenzen)

Produktnummer ABIN1043029

Kurzübersicht für Maus IgG2b Isotyp-Kontrolle (ABIN1043029)

Target

IgG2b

Klonalität

Monoklonal

Applikation

Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

MPC-11

Isotyp

IgG2b kappa

Wirt
120

66

2
Maus

Verwendungszweck

Mouse IgG2b Isotype Control Purified

Spezifität

This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.

Keine Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Purified by protein-A affinity chromatography.

Reinheit

> 95 % (by SDS-PAGE)

Immunogen

KLH-coupled trinitrophenol
Mouse IgG2b isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa Biotin

Mouse IgG2b isotype control (FITC)
FACS, IsoC Monoclonal MPC-11 IgG2b kappa FITC

Mouse IgG2b isotype control
FACS, ELISA Monoclonal A-1 IgG2b unconjugated

Mouse IgG2b isotype control (PerCP)
FACS, IsoC Monoclonal MPC-11 IgG2b kappa PerCP

Mouse IgG2b isotype control (APC)
FACS, IsoC Monoclonal MPC-11 IgG2b kappa APC

Mouse IgG2b isotype control (PE)
FACS, IsoC Monoclonal MPC-11 IgG2b kappa PE

Mouse IgG2b isotype control (FITC)
FACS, ELISA Monoclonal A-1 IgG2b FITC

Mouse IgG2b isotype control (PE)
FACS, ELISA Monoclonal A-1 IgG2b PE

Mouse IgG2b isotype control (iFluor™700)
GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™700

Mouse IgG2b isotype control (iFluor™647)
GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™647

Applikationshinweise

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
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Smed-Sörensen, Moll, Cheng, Loré, Norlin, Perbeck, Moody, Spetz, Sandberg: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).

Im, Tapinos, Chae, Illarionov, Besra, DeVries, Modlin, Sieling, Rambukkana, Porcelli: "Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5226-35, (2006) (PubMed).

Gupta, Wang, Vinson, Dziarski: "Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14012-20, (1999) (PubMed).
Target

IgG2b

Andere Bezeichnung

IgG2b kappa

Substanzklasse

Antibody