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Ac-DEVD-CHO (Caspase-3 Inhibitor)

Name: Ac-DEVD-CHO (Caspase-3 Inhibitor)
SKU: ABIN2690899
Availability: InStock

Func, InhA, BR

(2 Referenzen)

Produktnummer ABIN2690899

109,54 €

Zzgl. Versandkosten 20,00 € und MwSt

1 mg

Lieferung nach: Deutschland

Lieferung in 12 bis 15 Werktagen

Kurzübersicht für Ac-DEVD-CHO (Caspase-3 Inhibitor) (ABIN2690899)

Applikation

Functional Studies (Func), Inhibition Assay (InhA), Blocking Reagent (BR)

Verwendungszweck

The tetrapeptide inhibitor can be used to identify and quantify the Caspase-3 activity in apoptotic cells, and to study events downstream of Caspase-3 activation.

Sequenz

Acetyl-Asp-Glu-Val-Asp-CHO

Produktmerkmale

Members of the ICE/CED-3 cysteine protease family have key roles in inflammation and mammalian apoptosis. The ICE family member Caspase-3 (also known as CPP32, Yama, apopain) is activated early in apoptosis and appears to be involved in the proteolysis of several important molecules, including poly (ADP ribose) polymerase (PARP). Activated Caspase-3 cleaves PARP from its 116 kD to an 85 kD residual fragment. The cleavage site in PARP is C-terminal to Asp-216. The upstream sequence of the cleavage site, DEVD (Asp-Glu-Val-Asp), is utilized as a basis for the highly specific Caspase-3 substrate, Ac-DEVD-AMC and Caspase-3 inhibitor, Ac-DEVD-CHO. Ac-DEVD-CHO is a synthetic tetrapeptide inhibitor for Caspase-3 (CPP32) and contains the amino acid sequence of the PARP cleavage site. The tetrapeptide inhibitor can be used to identify and quantify the Caspase-3 activity in apoptotic cells, and to study events downstream of Caspase-3 activation.

Aufreinigung

Purified

Formel

C20H30N4O11

Löslichkeit

DMSO
Geldanamycin- FITC
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Geldanamycin
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Radicicol
Inh 12772-57-5

Geldanamycin- Biotin
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Herbimycin A
Inh 70563-58-5 [(2R,3S,5S,6R,7S,8E,10S,11S,12E,14E)-2,5,6,11-tetramethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

17-GMB-APA-GA
Inh [(3R,5S,6R,7S,10S,11S)-21-[3-[4-(2,5-dioxopyrrol-1-yl)butanoylamino]propylamino]-6-hydroxy-5,11-dimethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

TEMPO
Inh 2564-83-2 2,2,6,6-Tetramethylpiperidine 1-oxyl, 2,2,6,6-Tetramethyl-1-piperidinyloxy, free radical, (2,2,6,6-Tetramethyl-1-piperidinyl)oxidanyl, 1,1,5,5-tetramethylpentamethylene nitroxide, 2,2,6,6-Tetramethylpiperidine-1-oxyl

Triacsin C
Inh 76896-80-5 (3E)-1-Oxo-3-[(2E,4E,7E)-2,4,7-undecatrien-1-ylidene]triazane

TPCK
Inh 402-71-1 N-(4-Chloro-3-oxo-1-phenyl-2-butanyl)-4-methylbenzenesulfonamide

Staurosporine
Inh 62996-74-1 (2S,3R,4R,6R)-3-Methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-16-one

Kommentare

Spectrofluorometric analysis of Caspase-3 activity. Lysates were prepared from Daudi B cells untreated (left panel)) or treated with anti-human Fas antibody, clone DX2 (Cat. No. 555670) and Protein G (middle and right panels) for 4 hours to induce apoptosis. Cells were incubated with the Ac-DEVD-AMC, Caspase-3 substrate (left and middle panels) or the substrate and the Ac-DEVD-CHO inhibitor (right panel) and analyzed by spectrofluorometry. Left panel: Lysates from untreated cells did not emit fluorescence, indicating that the substrate was not cleaved and hence Caspase-3 activity was absent. Middle panel: Lysates from cells treated with anti-Fas mAb cleaved the substrate, indicating that presence of Caspase-3 activity. Right panel: Fluorescence was not emitted in lysates from cells treated with anti-Fas mAb when both the inhibitor and substrate were added, indicating that Caspase-3 activity was blocked. The addition of Protein G enhances the ability of clone DX2 to induce apoptosis, presumably by cross-linking Fas receptors.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Powder

Rekonstitution

Reconstitute in 1 mLDMSO to yield 1 mg/mL peptide in DMSO.

Buffer

Lyophilized powder

Lagerung

-20 °C

Informationen zur Lagerung

Store undiluted at -20°C. Reconstitute the substrate before use. Reconstitute in 1 ml DMSO to yield 1 mg/ml peptide in DMSO. Store the reconstituted substrate at -20°C for up to 1-2 months and avoid repeated freeze-thaw cycles, which greatly alter product stability.
Patel, Gores, Kaufmann: "The role of proteases during apoptosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 10, Issue 5, pp. 587-97, (1996) (PubMed).

Nicholson, Ali, Thornberry, Vaillancourt, Ding, Gallant, Gareau, Griffin, Labelle, Lazebnik: "Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis." in: Nature, Vol. 376, Issue 6535, pp. 37-43, (1995) (PubMed).
Molekulargewicht

502 Da