HSV1/2 IgM ELISA Kit
Dieses Human HSV1/2 IgM ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human HSV1/2 IgM zu quantifizieren.
Produktnummer ABIN7666555
Kurzübersicht für HSV1/2 IgM ELISA Kit (ABIN7666555)
Target
Alle HSV1/2 IgM Produkte
HSV1/2 IgM
(Anti-Herpes Simplex Virus Type 1/2 IgM (HSV1/2 IgM))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Indirect ELISA
Applikation
ELISA
Proben
Plasma, Serum
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Verwendungszweck
- anti-HSV 1+2 IgM assay is a solid-phase immunoanalytical test. T
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Marke
- ELISA-VIDITEST
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Analytische Methode
- Semi-Quantitative
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Bestandteile
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- ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
- 1.3 mL Positive control human serum, r.t.u. PC 1 vial
- 2.0 mL Calibrator (human serum), r.t.u. CAL 1 vial
- 13 mL Anti-human IgM animal antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
- 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- 2 mL RF-sorbent (anti-human IgG) 25 x concentrated RF SORB 25x 1 vial
- Instruction manual
- Quality Control Certificate
- 1) r.t.u., ready to use / mailto:info@vidia.cz
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Benötigtes Material
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Pre-coated
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Protokoll
- anti-HSV 1+2 IgM assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a native antigens containing immunodominant epitopes of HSV 1 and 2. Anti-HSV antibodies from samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away those that formed complexes with the antigens are later on recognised by animal anti-human IgM antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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- HSV1/2 IgM (Anti-Herpes Simplex Virus Type 1/2 IgM (HSV1/2 IgM))
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Andere Bezeichnung
- HSV1+2 IgM
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Substanzklasse
- Antibody
Target Alle HSV1/2 IgM Produkte
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