EXOS Antikörper (cleaved)
Es gibt 1 Publikation für dieses Produkt. Der Huhn Polyklonal anti-EXOS Antikörper wird verwendet zum Nachweis von EXOS in Proben von Pseudomonas aeruginosa. Er wurde validiert für WB.
(1 reference)
Produktnummer ABIN93557
598,15 €
Zzgl. Versandkosten 20,00 € und MwSt
100 μL
Lieferung nach:
Deutschland
Lieferung in 3 bis 5 Werktagen
Kurzübersicht für EXOS Antikörper (cleaved) (ABIN93557)
Target
EXOS
(ExoS Gene Product (EXOS))
Reaktivität
Pseudomonas aeruginosa
Wirt
Huhn
Klonalität
Polyklonal
Applikation
Western Blotting (WB)
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Bindungsspezifität
- cleaved
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Verwendungszweck
- Anti-ExoS | Exoenzyme S
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Kreuzreaktivität (Details)
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Pseudomonas aeruginosa
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Homologie
- Pseudomonas aeruginosa
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Aufreinigung
- Purified, total IgY
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Immunogen
- amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
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Isotyp
- IgY
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Applikationshinweise
- 1 : 5000 (WB)
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, (2012) (PubMed).
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- EXOS (ExoS Gene Product (EXOS))
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Andere Bezeichnung
- Exoenzyme S (ExoS)
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Hintergrund
- Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.
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Molekulargewicht
- 48 kDa
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UniProt
- Q51449
Target
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