CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays
ChIC, CUT&RUN
20 X
(4 Referenzen)Kurzübersicht für CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays (ABIN6950951)
Target
Nuclease
Applikation
Chromatin Immunocleavage (ChIC), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
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Wirt
- Escherichia coli (E. coli)
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Verwendungszweck
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This construct of Protein A and G fused to Micrococcal Nuclease is useful in performing CUT&RUN.
The pAG-MNase fusion enzyme may be used in conjunction with any of our CUT&RUN Product Sets.
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Marke
- CUTANA™
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Produktmerkmale
- Recombinantly produced in E. coli, CUTANA™ pAGMNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUTANA pAG-MNase contains a C-terminal 6xHis epitope tag.
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Applikationshinweise
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This product is sufficient to perform 50/250 CUT&RUN reactions.
Recommended use: 2.5 μL of the supplied enzyme into a 50 μL CUT&RUN reaction (20X dilution). -
Kommentare
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CUTANA pAG-MNase, the essential reagent for ChIC/CUT&RUN workflows:
- First-in-class commercial product for ChIC/CUT&RUN assays
- Optimized fusion of Proteins A and G with Micrococcal Nuclease (pAG-MNase) enables direct compatibility with a broad range of antibody isotypes
- 50 and 250 reaction pack sizes, enabling greater experimental throughput
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 20 X
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Buffer
- Provided as a 20X stock in 10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50 % glycerol.
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Handhabung
- Avoid freeze-thaw cycles
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
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Haltbarkeit
- 12 months
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: "Improved CUT&RUN chromatin profiling tools." in: eLife, Vol. 8, (2019) (PubMed).
: "Targeted in situ genome-wide profiling with high efficiency for low cell numbers." in: Nature protocols, Vol. 13, Issue 5, pp. 1006-1019, (2019) (PubMed).
: "An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites." in: eLife, Vol. 6, (2018) (PubMed).
: "ChIC and ChEC; genomic mapping of chromatin proteins." in: Molecular cell, Vol. 16, Issue 1, pp. 147-57, (2004) (PubMed).
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: "Improved CUT&RUN chromatin profiling tools." in: eLife, Vol. 8, (2019) (PubMed).
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- Nuclease
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Molekulargewicht
- 44 kDa
Target
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