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Protease S. aureus

1 mg/mL
Produktnummer ABIN5706431
  • Aufreinigung
    Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay.
    Biological Activity Comment
    880 units/mg
  • Applikationshinweise

    Application Note: Protease S. aureus has shown that one unit causes a change of 0.001 A280 nm per minute at 37° C at  pH 7.8 using casein as a substrate. Specific conditions for reactivity should be optimized by end user.

    Kommentare

    Synonyms: Glutamyl endopeptidase,Endoproteinase Glu-C, Staphylococcal serine proteinase, V8 protease, V8 proteinase

    Background: Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum  pH 's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay. Protease S. aureus is ideal for investigators involved in enzyme and infectious diease research.

    Gene Name: sspA

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1 mg/mL
    Buffer

    Buffer: None

    Stabilizer: None

    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C,-20 °C
    Haltbarkeit
    12 months
  • Gen-ID
    1280
    UniProt
    P0C1U8
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