EBV EA(D) IgA ELISA Kit
Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human EBV EA(D) IgA.
Produktnummer ABIN7666557
Kurzübersicht für EBV EA(D) IgA ELISA Kit (ABIN7666557)
Target
EBV EA(D) IgA
(Anti-Epstein-Barr Virus Early Antigen (D) IgA (EBV EA(D) IgA))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Indirect ELISA
Applikation
ELISA
Proben
Serum
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Verwendungszweck
- anti-EA (D) EBV IgA - EL ISA kit for detection of IgA antibodies to diffusion component of an early antigen (EA (D)) Epstein-Ba rr virus (EBV) in human sera.
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Marke
- ELISA-VIDITEST
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Analytische Methode
- Semi-Quantitative
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Bestandteile
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- ELISA break-away strips in a frame coated with the s pecific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
- 1.3 mL Positive control serum, r.t.u. CTRL + 1 vial
- 1.3 mL Negative control serum, r.t.u. CTRL - 1 vial
- 13 mL Anti-human IgA antibodies labelled with horseradish peroxidase (anti-IgA Px-conjugate), r.t.u. CONJ 1 vial
- 55 mL Wash buffer concentrate, 10x concentrate d WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
- 13 mL Stop solution, r.t.u. STOP 1 vial
- Sealable pouch for unused strips Instruction manual
- Quality control certificate 1) ready to use Chromogenic substrate TMB is compatible an d interchangeable between ELISA-VIDITEST kits which contain TMB and not with other Chrom ogenic substrates TMB-O, TMB-BF. 1 http://www.vidia.cz/
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Benötigtes Material
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Pre-coated
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Protokoll
- anti-EA (D) EBV IgA assay i s a solid-phase immunoanalytical test. The polystyrene strips are coated with a recombinant antigen that b ears immunodominant epitopes of EA protein. The anti- EA (D) antibodies, if present in the serum, bind t o the immobilized antigens. The antibodies that do not bind are washed away and those that formed compl exes with the antigens are later on recognised by animal anti-human IgA antibodies labelled with horserad ish peroxidase. The presence of labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Target
- EBV EA(D) IgA (Anti-Epstein-Barr Virus Early Antigen (D) IgA (EBV EA(D) IgA))
Andere Bezeichnung
- EA-D Epstein-Barr Virus IgA
Substanzklasse
- Antibody
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