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DDX11L8 Protein (AA 1-907) (Strep Tag)

Dieses Recombinant DDX11L8-Protein wird in Cell-free protein synthesis (CFPS) produziert.
Produktnummer ABIN3078685
ab
15.754,29 €
Zzgl. Versandkosten 20,00 €, Trockeneispauschale 20,00 € sowie MwSt
250 μg
Lieferung nach: Deutschland
Lieferung in 51 bis 61 Werktagen

Kurzübersicht für DDX11L8 Protein (AA 1-907) (Strep Tag) (ABIN3078685)

Target

DDX11L8 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 Like 8 (DDX11L8))

Protein-Typ

Recombinant

Spezies

Human

Quelle

  • 1
Cell-free protein synthesis (CFPS)

Applikation

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Reinheit

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse

custom-made
  • Proteineigenschaft

    AA 1-907

    Aufreinigungstag / Konjugat

    Dieses DDX11L8 Protein ist gelabelt mit Strep Tag.

    Sequenz

    MANETQKVGA IHFPFPFTPY SIQEDFMAEL YRVLEAGKIG IFESPTGTGK SLSLICGALS WLRDFEQKKR EEEARLLETG TGPLHDEKDE SLCLSSSCEG AAGTPRPAGE PAWVTQFVQK KEERDLVDRL KVEQARRKQR EERLQQLQHR VQLKYAAKRL RQEEEETENL LRLSREMLET GPEAERLEQL ESGEEELVLA EYESDEEKKV ASGHRVDEDE DDLEEEHITK IYHCSRTHSQ LAQFVHEVKK SPFGKDVRLV SLGSRQNLCV NEDVRSLGSV QLINDRCVDM QRSRHEKKKG AEEEKPKRRR QEKQAACPFY NHEQMGLLRD EALAEVKDME QLLALGKEAR ACPYYRSRLA IPAAKLVVLP YQMLLHAATR QAAGIRLQDQ VVIIDEAHNL IDTITGMHSV EVSGSQLCQA HSQLLQYMER YGKRLKAKNL MYLKQILYLL EKFVAVLGGN IKQNPNTQSL SQTGTELKTI NDFLFQSQID NINLFKVQRY CEKSMISRKL FGFTERYGAV FSSREQPKLA GFQQFLQSLQ PRTTEALAAP ADESQASVPQ PASPLMHIEG FLAALTTANQ DGRVILSRQG SLSESTLKFL LLNPAVHFAQ VVKECRAVVI AGGTMQPVSD FRQQLLACAG VEAERVVEFS CGHVIPPDNI PLVICSGISN QPLEFTFQKR DLPQMMDEVG RILCNLCGVV SGGVVCFFSS YEYLRQVHAH WEKGGLLGRL AARKKIFQEP KSAHQVEQVL LAYSRCIQAC GQERGQVTEA LLLSVVGGKM SEGINFSDNL GRCVVMVGMP FPNIRSAELQ EKMAYLDQTL PRAPGQAPPG KALVENLCMK AVNQSIGRAI RHQKDFASIV LLDQRYARPP VLAKLPAWIR ASVEVKATFG PAIAAVQKFH REKSASS
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    Produktmerkmale

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Aufreinigung

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • Applikationshinweise

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    Handhabung

    Avoid repeated freeze-thaw cycles.

    Lagerung

    -80 °C

    Informationen zur Lagerung

    Store at -80°C.

    Haltbarkeit

    12 months
  • Target

    DDX11L8 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 Like 8 (DDX11L8))

    Andere Bezeichnung

    DDX11L8

    Hintergrund

    Putative ATP-dependent RNA helicase DDX11-like protein 8 (EC 3.6.4.13) (DEAD/H box protein 11-like 8),FUNCTION: Putative DNA helicase. {ECO:0000250}.

    Molekulargewicht

    101.8 kDa

    UniProt

    A8MPP1
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