Cell Lysis Buffer

Produktnummer ABIN5706385

Hersteller Produkt- Nr.: mb-078-0015

Kurzübersicht für Cell Lysis Buffer (ABIN5706385)

Applikation: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Hersteller Produkt- Nr.: mb-078-0015

Hersteller: Rockland

Verwendungszweck: Cell Lysis Buffer

Aufreinigung: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise:

Application Note: The buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.

ChIP Dilution: User Optimized

Western Blot Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 X

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store cell lysis buffer at -20° C prior to opening. Buffer is stable for up to 2 years stored at -20°C. It is recommended to aliquot and freeze buffer for extended usage. Store thawed buffer at 2-8°C for no longer than 2 weeks. This product MAY be stable for up to two (2) weeks if properly stored and handled.

Haltbarkeit: 2 weeks

Antigendetails

Hintergrund:

Synonyms: Cell Lysis Buffer

Background: Cell Lysis Buffer is used to lyse cells under non-denaturing conditions. If recommended add 1 mM PMSF or other protease inhibitor immediately before use. Keep all reagents and lysates in ice. For adherent cells add 400μL of buffer per 10cm flask once