Functional Bead Conjugation Buffer Set

Produktnummer ABIN2690896

Kurzübersicht für Functional Bead Conjugation Buffer Set (ABIN2690896)

Applikation: Immunoassay (IA), Flow Cytometry (FACS)

Produktdetails

Produktmerkmale: The Functional Bead Conjugation Buffer Set contains the supporting reagents necessary to covalently link water-soluble proteins to the surface of functional beads . The beads, once linked to a protein, can be used as a capture matrix for analytes of interest . The Functional Bead Conjugation Buffer Set contains enough material for 15 coupling reactions . Each coupling reaction yields approximately 3 - 6 ×10^6 beads which is enough material to run 500 or 1000 tests at a concentration of 6,000 beads per well . For a 500 test conjugation reaction 90 μg of protein is needed, and for a 1000 test conjugation reaction 180 μg of protein is needed . After covalently linking protein to the beads, it is recommended that a test be performed to confirm the success of the reaction . For that purpose, BD Biosciences offers three different phycoerythrin (PE) Functional Bead Ig Detectors that can be used to confirm the presence of antibody covalently linked to the beads.

Bestandteile: Coupling buffer and storage buffer

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C