ProXtract B™ Extraction Buffer

Produktnummer ABIN1607929

Hersteller Produkt- Nr.: mb-087-0100

Kurzübersicht für ProXtract B™ Extraction Buffer (ABIN1607929)

Applikation: Western Blotting (WB)

Produktdetails

Hersteller Produkt- Nr.: mb-087-0100

Hersteller: Rockland

Marke: ProXtract B™

Produktmerkmale: ProXtract B™ is ready-to-use as a working 1X solution and requires no further dilution. This buffer can be stored and used at room temperature. ProXtract B™ Extraction Buffer is gentle and has a high efficiency of protein recovery. It is compatible with chelating agents, reducing agents, protease inhibitors, cocktail inhibitors and chaotropic salts. There is no interference with downstream purification processes. Other applications may require detergents or other additional components. Synonyms: ProXtract B™ Extraction Buffer, Protein Extraction Buffer, Bacterial Protein Extraction Reagent, Bacterial Cell Lysis Buffer

Endotoxin-Niveau: Low Endotoxin : No

Anwendungsinformationen

Applikationshinweise: ProXtract B™ Buffer is suitable for protein extraction and easily scalable. After protein induction, bacterial cells are pelleted by ultracentrifugation at 4000 rpm during 10 minutes at 4°C. The cell pellet is resuspended with ProXtract buffer (usually 20-30 ml per gram of wet cells) with incubation at least 20 mins on an orbital shaker at 18-26°C. Centrifugation is then done at 12000 rpm for 20 minutes at 4°C. The supernatant contains solubilized proteins and is ready to be analyzed by SDS-PAGE, Western-Blot or purified through downstream purification processes.
Western Blot Dilution: 2-5µl

Kommentare:

ProXtract B Extraction Buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C

Informationen zur Lagerung: Store container at room temperature (18 °C to 26 °C) prior to opening. If desired, the solution may be stored at 4 °C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18 °C to 26 °C) to restore solubility of some salts.

Haltbarkeit: Expiration date is six (6) months from date of opening.