CHAPS Buffer

Produktnummer ABIN1059064

Kurzübersicht für CHAPS Buffer (ABIN1059064)

Applikation: Immunoprecipitation (IP)

Produktdetails

Produktmerkmale: Strong cell lysis and protein extraction capacity while preserving protein conformation and protein-protein interaction for co-immunoprecipitation (Co-IP) analysis.
Effective as a stand alone reagent for cell lysis and protein extraction.
Contains a non-amine buffer enabling cross-linking with NHS-ester derivatives.
Minimal interference with Bradford and Lowry protein assays.
60 mL volume: Suitable for two hundred 10-cm culture plates, or 600 wells in 6-well dishes.

Anwendungsinformationen

Kommentare:

Properties of CHAPS Lysis and Immunoprecipitation BufferCHAPS is a zwitterionic detergent with strong membrane dissolution capacity while maintaining protein conformation and protein-protein interaction. CHAPS reagent provides an effective stand alone buffer for cell lysis and protein extraction as well as a reagent suitable for downstream immunoprecipitation-pull down approaches. The standard FIVEphoton Biochemicals CHAPS reagent is provided without calcium chloride or EDTA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 %

Buffer: CHAPS based cell lysis and immunoprecipitation buffer containing 0.5% CHAPS, pH 8.0.

Lagerung: 4 °C