anti-LAMP2 (LAMP2) Antikörper

Human Lysosomal-Associated Membrane Protein 2 (LAMP2) Interaktionspartner

1. basal and inducible regulation of LAMP2A, and consequently Chaperone-mediated autophagy activity, by NFE2L2, is reported.

2. the first two DD heterozygote female probands who harbor novel multi-exon LAMP2 deletions, are reported.

3. LAMP-2 defciency is associated with Small-vessel vasculopathy Due to Aberrant Autophagy.

4. our results unravel a paradigm in which intracellular protein LAMP2a regulates TAMs activation to promote tumor progression. And this LAMP2a-dependent mechanism might partly illustrate how tumor cells utilize microenvironment to domesticate TAMs and suppress anti-tumor immunity.

5. we showed that the activation of ferroptosis by erastin increased the levels of lysosome-associated membrane protein 2a to promote chaperone-mediated autophagy (CMA), which, in turn, promoted the degradation of GPX4. Importantly, inhibition of CMA stabilized GPX4 and reduced ferroptosis. Our results suggest that activation of CMA is involved in the execution of ferroptosis.

6. that CSF lamp2 concentrations may be decreased in female LRRK2 Parkinson's disease patients compared to healthy individuals with or without LRRK2 mutations

7. the results presented here showed that Lamp-2 deficiency in human lymphoblastoid cells did not modify the steady-state levels or the degradation of several protein substrates reported as selective substrates of the chaperone-mediated autophagic pathway pathway.

8. These findings reveal a STX17-independent autophagic fusion mechanism in human CMs, providing an explanation for cardiomyopathy in Danon patients and a foundation for targeting defective LAMP-2B-mediated autophagy to treat this patient population.

9. A missense mutation in the LAMP2 was identified in two siblings with dilated cardiomyopathy.

10. Male patients showed completely absent LAMP-2 expression in muscle. In contrast, female patients showed decreased LAMP-2 expression, which is suggested to reflect LAMP-2 haploinsufficiency due to a heterozygous null mutation. In conclusion, Danon disease is an extremely rare muscular disorder in Japan

11. Interruption of LAMP-2 expression did not result in an increase of the steady-state protein levels of DJ-1 /PARK7, as it would have been expected. No change in DJ-1 /PARK7 protein levels were observed upon inhibition of lysosomal function with NH4Cl or NH4Cl plus leupeptin, or after activation of CMA by serum starvation for 24h.

12. Downregulation of chaperone-mediated autophagy (CMA) activity by LAMP2A knockdown significantly inhibited the growth and metastasis in vivo and in vitro, while upregulation of CMA activity by LAMP2A overexpression had the opposite effect.

13. Knockdown of key autophagy proteins in combination with sunitinib showed similar effect as chloroquine. Sunitinib also induced lysosomal membrane permeabilization, which further increased in the presence of chloroquine or knockdown of lysosome-associated membrane protein (LAMP2). Both combinations led to cell death

14. Results demonstrated that LAMP2 expression levels correlated with tumor histological differentiation and TNM stages.

15. GnT-IVa may contribute to the malignancy of choriocarcinoma by promoting cell adhesion, migration and invasion through glycosylation of integrin beta1 and LAMP-2.

16. Since effective regimens are readily available timely psychiatric evaluation is warranted in all newly diagnosed subjects with LAMP2 mutations, regardless of whether they show the typical Danon disease medical (cardiac) symptoms or not.

17. The authors show here that human LAMP1 and LAMP2 bind cholesterol in a manner that buries the cholesterol 3beta-hydroxyl group; they also bind tightly to NPC1 and NPC2 proteins that export cholesterol from lysosomes.

18. identified LAMP-2 as an endocytic receptor on monocyte-derived dendritic cells (MoDC) that routes cargo into unusual Ag processing pathways, which reduces surface expression of Ag-derived peptides while selectively enriching Ag within immunogenic exosome; this novel pathway has implications for the initiation of immune responses both locally and at distant sites

19. downregulation of FUT1, which leads to the perinuclear localization of LAMP-1 and 2, is correlated with increased rate of autophagic flux by decreasing mTOR signaling and increasing autolysosome formation.

20. The results provide a new insight that LAMP-2 contributes to the ROS clearance and cell death induced by Zn(2+) treatment, which would help us to get a better understanding of Zn(2+)-induced toxicity in respiratory system.

Mouse (Murine) Lysosomal-Associated Membrane Protein 2 (LAMP2) Interaktionspartner

1. basal and inducible regulation of LAMP2A, and consequently Chaperone-mediated autophagy activity, by NFE2L2, is reported.

2. High Lamp2 expression is associated with kidney fibrosis.

3. Interruption of LAMP-2 expression did not result in an increase of the steady-state protein levels of DJ-1 /PARK7, as it would have been expected. No change in DJ-1 /PARK7 protein levels were observed upon inhibition of lysosomal function with NH4Cl or NH4Cl plus leupeptin, or after activation of CMA by serum starvation for 24h.

4. Lysosomal p38 MAPK directly phosphorylates LAMP2A at T211 and T213, which causes its membrane accumulation and active conformational change, activating chaperone-mediated autophagy.

5. Both LAMP-1 and LAMP-2 are involved in C2C12 myotube formation and LAMP-2 may contribute dominantly to it.

6. The results presented here show the major role of LAMP-2 in caveolin traffic and membrane repair and consequently in T. cruzi invasion.

7. The up-regulation of Rab11, Rab7, or RILP, but not its truncated form RILP-C33, rescued LAMP2A-defective trafficking in cystinosis, whereas dominant-negative Rab11 or Rab7 impaired LAMP2A trafficking.

8. LAMP-2 in the central nervous system has a possible role in the degradation of the various macromolecules in lysosomes and an additional function concerning protection from oxidative stress, especially in the substantia nigra.

9. The absence of LAMP-2 in murine brain led to inflammation and abnormal behavior, including motor deficits and impaired learning.

10. Lamp-2 deficiency prevents high-fat diet-induced obese diabetes via enhancing energy expenditure.

11. These results suggest that VPS35 deficiency or mutation promotes Parkinson Disease (PD) pathogenesis, and reveals a crucial pathway, VPS35-Lamp2a-alpha-synuclein, to prevent PD pathogenesis.

12. The chaperone-mediated autophagy receptor LAMP2A is downregulated in cystinotic cells.

13. deletion of the gene encoding LAMP-2A in T cells caused deficient in vivo responses to immunization or infection with Listeria monocytogenes.

14. LAMP proteins retain TAPL on the limiting membrane of endosomes and prevent its sorting to intraluminal vesicles.

15. Restoration of lysosome-associated membrane protein-2 levels synergizes with partial BECLIN-1 knockdown to restore autophagosome processing and to attenuate cell death after hypoxia-reoxygenation.

16. results suggest that LAMP-2, its luminal domain in particular, plays a critical role in endosomal cholesterol transport and that this is distinct from the chaperone-mediated autophagy function of LAMP-2

17. Using LAMP1/2 knock out cells the authors show that these two proteins are important for Trypanosoma cruzi infection of host cells, both in entrance and intracellular development, conceivably by being the major source of sialic acid for Trypanosoma cruzi.

18. LAMP-2 has a role in lysosome biogenesis and autophagy

19. Danon's disease resulted from a yet undescribed deletion in exon 7 of lamp-2 gene.

20. Sequencing of LAMP-2 gene showed a novel S157X mutation in exon 4.