Glycan Array 100

Produktnummer ABIN4973898

Kurzübersicht für Glycan Array 100 (ABIN4973898)

Nachweismethode: Fluorometric

Applikation: Antibody Array (AA)

Proben: Plasma, Cell Culture Supernatant, Purified Protein, Serum, Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Produktdetails

Methodentyp: Sandwich ELISA

Verwendungszweck: Detection of binding partners for 100 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.

Spezifität: Beta-Glc-, beta-Gal-, alpha-Man-, alpha-Fuc-, alpha-Rha-, beta-GlcNAc-, beta-GalNAc-, Tobramycin, Gal-beta-1,3-GlcNAc-beta-, Gal-alpha-1,3-Gal-beta-1,3-GlcNAc-beta-, Neu5Ac-alpha-2,3-Gal-beta-1,3-GlcNAc-beta-, Neu5Ac-alpha-2,6-Gal-beta-1,3-GlcNAc-beta-, Neu5Gc-alpha-2,3-Gal-beta-1,3-GlcNAc-beta-, Neu5Gc-alpha-2,6-Gal-beta-1,3-GlcNAc-beta-, Gal-beta-1,3-(Fuc-alpha-1,4)-GlcNAc-beta- [Lewis A], Gal-beta-1,4-Glc-beta-, Gal-alpha-1,3-Gal-beta-1,4-Glc-beta-, Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-, GlcNAc-beta-1,3-Gal-beta-1,4-Glc-beta-, GalNAc-beta-1,3-Gal-beta-1,4-Glc-beta-, Neu5Ac-alpha-2,3-Gal-beta-1,4-Glc-beta-, Neu5Ac-alpha-2,6-Gal-beta-1,4-Glc-beta-, Neu5Gc-alpha-2,3-Gal-beta-1,4-Glc-beta-, Neu5Ac-alpha-2,6-Gal-beta-1,4-Glc-beta-, Gal-beta-1,4-(Fuc-alpha-1,3)-Glc-beta-, GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-, GlcNAc-beta-1,6-GlcNAc-beta-, 4-P-GlcNAc-beta-1,4-Man-beta-, Glc-alpha-1,2-Gal-alpha-1,3-Glc-alpha-, Gal-beta-1,3-GalNAc-alpha-, Gal-beta-1,4-GlcNAc-beta-, Gal-beta-1,4 -(Fuc-alpha-1,3)-GlcNAc-beta- [Lewis X], Neu5Ac-alpha-2,3-Gal-beta-1,4-(Fuc-alpha-1,3)-GlcNAc-beta- [Sialyl Lewis X], Neu5Ac-alpha-2,3-Gal-beta-1,3 -(Fuc-alpha-1,4)-GlcNAc-beta- [Sialyl Lewis A], Neu5Gc-alpha-2,3-Gal-beta-1,3-(Fuc-alpha-1,4)-GlcNAc-beta- [Sialyl Lewis A], Gal-alpha-1,4-Gal-beta-1,3-GlcNAc-beta-, Gal-beta-1,4-GlcNAc-beta-1,3-Gal-beta-1,4-Glc-beta- [LNnT], GlcA-beta-1,4-GlcNAc-alpha-1,4-GlcA-beta-, GlcNAc-beta-1,6-(Gal-beta-1,3)-GalNAc-alpha-O-Ser, Neu5Ac-alpha-2,3Gal-beta-1,4-(6S)GlcNAc-beta-, GalNAc-beta-1,4-GlcNAc-beta-, Neu5Ac-alpha-2,8-Neu5Ac-alpha-2,3-Gal beta-1,4-Glc-beta-, Neu5Gc-alpha-2,8-Neu5Ac-alpha-2,3-Gal-beta-1,4-Glc-beta-, GalNAc-alpha-1,3-(Fuc-alpha-1,2)-Gal-beta-1,4-Glc-beta- [Blood A antigen tetrose], GlcNAc-beta-1,2-Man-alpha-, Neu5Ac-alpha-2,3-Gal-beta-, Gal-beta-1,3 -GalNAc-beta-1,3-Gal-beta-, Glc-alpha-1,2-Gal-alpha-, Gal-beta-1,4-(Fuc-alpha-1,3)-GlcNAc-beta-1,3-Gal-beta-, Neu5Ac-alpha-2,3-Gal-beta-1,4-(Fuc-alpha-1,3)-Glc-beta- [3-Sialyl-3-fucosyllactose/ F-SL], GlcNAc-beta-1,4-GlcNAc-beta-, beta-D-GlcA-, Gal-beta-1,4-(6S)GlNAc-beta-, GlcNAc-alpha-1,3-(Glc-alpha-1,2-Glc-alpha-1,2)-Gal-alpha-1,3-Glc-alpha-, Gal-beta-1,3-GalNAc-beta-1,4-(Neu5Gc-alpha-2,3)-Gal-beta-1,4-Glc-beta-, Sisomicin Sulfate, GalNAc-alpha-1,3-(Fuc-alpha-1,2)-Gal-beta- [Blood A antigen trisaccharide], Fuc-alpha-1,2-Gal-beta-1,4-GlcNAc-beta- [Blood H antigen trisaccharide], Gal-alpha-1,3-(Fuc-alpha-1,2)-Gal-beta- [Blood B antigen trisaccharide], Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-beta-1,3-Gal-beta-1,4-Glc-beta- [LNFP I], Fuc-alpha-1,2-Gal-beta-1,4-Glc-beta- [Blood H antigen trisaccharide], Gal-alpha-1,3-(Fuc-alpha-1,2)-Gal-beta-1,4-Glc-beta- [Blood B antigen tetrasaccharide], (Fuc-alpha-1,2)-Gal-beta-1,4-(Fuc-alpha-1,3)-GlcNAc-beta- [ Lewis Y], (Fuc-alpha-1,2)-Gal-beta-1,3-(Fuc-alpha-1,4)-GlcNAc-beta- [ Lewis B], Gal-beta-1,3-(Fuc-alpha-1,4)-GlcNAc-beta-1,3-Gal-beta-1,4-(Fuc-alpha-1,4)-Glc-beta- [Lewis A], Gal-beta-1,3-GalNAc-beta-, Gal-beta-1,3-(Neu5Ac-alpha-2,6)-GalNAc-beta-, Neu5Ac-alpha-2,6-Gal-beta-1,3-GalNAc-beta-, Neu5Ac-alpha-2,6-Gal-beta-1,3-(Neu5Ac-alpha-2,6)-GalNAc-beta-, Neu5Ac-alpha-2,3-Gal-beta-1,3-(Neu5Ac-alpha-2,6)-GalNAc-beta-, Neu5Ac-alpha-2,6-(Neu5Ac-alpha-2,3)-Gal-beta-1,3-GalNAc-beta-, GalNAc-beta-1,4-(Neu5Ac-alpha-2,3)-Gal-beta-1,4-Glc-beta- [GM2], GalNAc-beta-1,4-(Neu5Ac-alpha-2,8-Neu5Ac-alpha-2,3)-Gal-beta-1,4-Glc-beta- [GD2], Gal-alpha-1,4-Gal-beta-1,4-GlcNAc-beta-, beta-D-Rha-, Glc-alpha-1,4-Glc-beta-, Glc-alpha-1,6-Glc-alpha-1,4-Glc-beta-, Maltotriose-beta-, Glc-alpha-1,6-Glc-alpha-1,6-Glc-beta-, Maltotetraose-beta-, GlcNAc-alpha-1,4-GlcA-beta-1,4-GlcNAc-alpha1,4-GlcA-beta-, Maltohexaose-beta-, Maltoheptaose-beta-, Acarbose-beta-, D-pentamannuronic acid-beta-, L-pentaguluronic acid-beta-, D-cellose-beta-, Gal-alpha-1,3-Gal-beta-, beta-1,4-Xylotetrose, Chitin-trisaccharide, KDN-alpha-2,8-Neu5Ac-alpha-2,3-Gal-beta-1,4-Glc-beta-, Neu5Ac-alpha-2,8-Neu5Gc-alpha-2,3-Gal-beta-1,4-Glc-beta-, Neu5Ac-alpha-2,8-Neu5Ac-alpha-2,8-Neu5Ac-alpha-2,3-Gal-beta-1,4-Glc-beta-, Neu5Ac-alpha-2,8-Neu5Ac-alpha-2,6-Gal-beta-1,4-Glc-, Gal-beta-1,3-GalNAc-beta-1,4-(Neu5Ac-alpha-2,3)-Gal-beta-1,4-Glc-beta-, Gentamicin Sulfate, Kanamycin sulfate, Geneticin Disulfate Salt (G418), Neomycin trisulfate, SGP

Produktmerkmale:

• High sensitivity and specificity
• Low sample volume (20-100 μL per array)
• Large dynamic range of detection
• Compatible with most sample types
• Test 4 samples on each slide
• Suitable for high-throughput assays

Bestandteile: Dialysis Vials
Labeling Reagent
Stop Solution
Glycan Array Glass Slide Assembly
Sample Diluent
20X Wash Buffer I
20X Wash Buffer II
Cy3 equivalent dye-conjugated Streptavidin
Slide Washer/Dryer
Adhesive device sealer
Labeling Buffer
Floating Dialysis Rack

Benötigtes Material: Detection antibodies of interest (For sandwich-based method only)
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Distilled or de-ionized water
1.5 mL Polypropylene microcentrifuge tubes
Gene microarray scanner or similar laser fluorescence scanner
Small plastic or glass containers
KCl, NaCl, KH2PO4 and Na2HPO4 (For label-based method only)
Beaker, stir plate and stir bar

Anwendungsinformationen

Applikationshinweise: This array will help researchers:
Identify the glycans binding partners in biological samples
Identify whether target proteins are carbohydrate binding proteins
Probe binding of viruses and whole cells to glycans1-3
Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.)
Profile the inflammatory immune response

Plattentyp: Glass Slide

Protokoll: Dry the glass slideBlock array surfacesIncubate samples (samples need to be biotinylated for the label-based approach)For the the sandwich-based principle, incubate with a detection antibody cocktailFor the label-based principle, incubate the labeled-streptavidin.Incubate with Cy3 Equivalent Dye-StreptavidinDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysis

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.