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AOX1/AOX2 Antikörper (C-Term)

Dieses Anti-AOX1/AOX2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von AOX1/AOX2 in WB. Geeignet für Arabidopsis thaliana, Lupinus luteus, Moss, Nicotiana tabacum, Pisum sativum und Potato. Dieses Primary Antibody wurde in 7+ Publikationen zitiert.
Produktnummer ABIN3197483

Kurzübersicht für AOX1/AOX2 Antikörper (C-Term) (ABIN3197483)

Target

AOX1/AOX2 (Alternative Oxidase 1/2 (AOX1/AOX2))

Reaktivität

Arabidopsis thaliana, Lupinus luteus, Moss, Nicotiana tabacum, Pisum sativum, Potato

Wirt

Kaninchen

Klonalität

Polyklonal

Applikation

Western Blotting (WB)
  • Bindungsspezifität

    C-Term

    Kreuzreaktivität (Details)

    No cross-reactivity with: Chlamydomonas reinhardtii

    Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 36-40 / 36-40 for Arabidopsis thaliana

    Aufreinigung

    serum

    Immunogen

    KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms 1 and 2 including Arabidopsis thaliana AOX1A (At3g22370) and AOX2 (At3g64210), Solanum lycopersicum Q7XBG9, Oryza sativa Q7XT33,
  • Applikationshinweise

    Recommended Dilution: 1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection(WB).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 200 µL of sterile water

    Handhabung

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Lagerung

    -20 °C
  • Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).

    Tsaniklidis, Delis, Nikoloudakis, Katinakis, Aivalakis: "Low temperature storage affects the ascorbic acid metabolism of cherry tomato fruits." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 84, pp. 149-57, (2014) (PubMed).

    Zubo, Potapova, Yamburenko, Tarasenko, Konstantinov, Börner: "Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria." in: Mitochondrion, Vol. 19 Pt B, pp. 222-30, (2014) (PubMed).

    Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).

    Kornfeld, Heskel, Atkin, Gough, Griffin, Horton, Turnbull: "Respiratory flexibility and efficiency are affected by simulated global change in Arctic plants." in: The New phytologist, Vol. 197, Issue 4, pp. 1161-72, (2013) (PubMed).

    Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

    Wang, Li, Liu, He, Bi: "Nitric oxide increases mitochondrial respiration in a cGMP-dependent manner in the callus from Arabidopsis thaliana." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, (2010) (PubMed).

  • Target

    AOX1/AOX2 (Alternative Oxidase 1/2 (AOX1/AOX2))

    Andere Bezeichnung

    Plant alternative oxidase 1 and 2 (AOX1/2)

    Hintergrund

    AGI Code: At3g22370
    Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.

    Molekulargewicht

    expected: 36-40 kDa, apparent: 36-40 for Arabidopsis thaliana

    UniProt

    Q7XBG9, Q7XT33, O22049, Q39219
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