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Western Blot Antikörper

Western-Blot (WB)-Antikörper sind für den Nachweis denaturierter Proteine optimiert und validiert, um klare, spezifische Banden bei der erwarteten Molekularmasse zu liefern. Verwenden Sie die untenstehenden Filter, um schnell primäre Antikörper zu finden, die zu Ihrem Zielprotein, Ihrer Spezies und Ihren experimentellen Anforderungen passen.

Mehr über Western Blot erfahren

Validierung von Western-Blot-Antikörpern

Antikörper für den Western Blot müssen Zielproteine unter denaturierenden Bedingungen zuverlässig nachweisen. Produkte in dieser Kategorie sind für WB-Anwendungen validiert und für die spezifische Detektion von Banden bei der erwarteten Molekularmasse ausgewählt. Verfügbare Validierungsdaten umfassen experimentelle Abbildungen, unabhängige Tests und Literaturreferenzen zur Unterstützung der Antikörperleistung.

Überlegungen zum Western Blot

Die Denaturierung von Proteinen während des Western Blots kann die Epitopstruktur verändern, wodurch die Auswahl geeigneter Antikörper entscheidend wird. Für WB validierte Antikörper sind darauf optimiert, linearisierte Epitope zu erkennen und unspezifische Bindung zu reduzieren.

Monoklonale Antikörper bieten hohe Spezifität und konsistente Ergebnisse, während polyklonale Antikörper durch die Erkennung mehrerer Epitope oft ein stärkeres Signal liefern. Rekombinante Antikörper kombinieren definierte Spezifität mit verbesserter Reproduzierbarkeit über Experimente hinweg.

Western Blot: Grundlagen, Protokolle und Troubleshooting

Mehr über Western Blot erfahren, einschließlich Protokollen und Troubleshooting

Verwenden Sie die untenstehenden Filter, um Western-Blot-Antikörper nach Zielprotein, Spezies und experimentellen Anforderungen einzugrenzen.

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530.699 Treffer

GAPDH Antikörper (C-Term)

GAPDH Reaktivität: Human WB, IF, IHC (p), FACS Wirt: Kaninchen Polyclonal RB16543 unconjugated

Western blot analysis of GDH Antibody (C-term ) 7873b in , , CEM cell line lysates (35 μg/lane).

Flow cytometric analysis of HepG2 cells using GDH Antibody (C-term )(bottom histogram) compared to a negative control cell (top histogram).

FACS

Confocal immunofluorescent analysis of GDH Antibody (C-term ) 7873b with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

All lanes : Anti-GDH Antibody (C-term ) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane.

Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GDH Antibody (C-term ) 7873b.

Formalin-fixed and paraffin-embedded human prostate carcinoma with GDH Antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

7 Abbildungen

1 reference

Produktnummer ABIN392354

434,77 €

Zzgl. Versandkosten 20,00 € und MwSt

400 μL (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 14 bis 17 Werktagen

Details

PCNA Antikörper (AA 53-196)

PCNA Reaktivität: Human, Maus, Ratte WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 7H4F8 unconjugated

Western blot analysis using PCNA mAb against HEK293 (1) and PCNA (AA: 53-196)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PCNA mouse mAb with DAB staining.

IHC

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

ELISA

Western blot analysis using PCNA mouse mAb against A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate.

Western blot analysis using PCNA mAb against human PCNA recombinant protein.

Flow cytometric analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple).

FACS

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PCNA mouse mAb with DAB staining.

IHC

7 Abbildungen

2 Referenzen

Produktnummer ABIN1724760

657,84 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 7 bis 10 Werktagen

Details

PCNA Antikörper (AA 53-196)

PCNA Reaktivität: Human, Affe WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 7H4F8 unconjugated

IHC

FACS

ELISA

Western blot analysis using PCNA mouse mAb against A431 (1), HEK293 (2), HeLa (3), HepG2 (4), Raji (5), MOLT4 (6), COS7 (7), and MCF-7 (8) cell lysate.

IHC

7 Abbildungen

2 Referenzen

Produktnummer ABIN1724759

657,84 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 7 bis 10 Werktagen

Details

PCNA Antikörper (AA 151-261)

PCNA Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Mouse spleen lysates probed with PCNA Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded human brain tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated (ABIN676583) at 1:200 followed by conjugation to the secondary antibody and DAB staining

6 Abbildungen

6 Referenzen

Produktnummer ABIN676583

120,76 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

Details

PCNA Antikörper (AA 1-261)

PCNA Reaktivität: Human WB, ELISA, IF, IP Wirt: Maus Monoclonal 1G7 unconjugated

PCNA monoclonal antibody (M02), clone 1G7 Western Blot analysis of PCNA expression in Hela .

Immunofluorescence of monoclonal antibody to PCNA on HeLa cell.

Detection limit for recombinant GST tagged PCNA is approximately 0.

ELISA

Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M02), clone 1G7.

Immunoprecipitation of PCNA transfected lysate using anti-PCNA monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PCNA MaxPab rabbit polyclonal antibody.

Western Blot detection against Immunogen (54.

7 Abbildungen

1 reference

Produktnummer ABIN562135

651,20 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μg

Lieferung nach: Deutschland

Lieferung in 8 bis 9 Werktagen

Details

PCNA Antikörper (AA 1-261)

PCNA Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal S1 unconjugated

PCNA monoclonal antibody (M04), clone S1 Western Blot analysis of PCNA expression in Hela S3 NE .

ELISA

PCNA monoclonal antibody (M04), clone S1.

Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M04), clone S1.

7 Abbildungen

1 reference

Produktnummer ABIN562136

651,20 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μg

Lieferung nach: Deutschland

Lieferung in 8 bis 9 Werktagen

Details

PCNA Antikörper

PCNA Reaktivität: Human WB, IHC, IF, IP, IHC (p), ICC, IHC (fro), FISH Wirt: Kaninchen Polyclonal unconjugated

WB Image PCNA antibody detects PCNA protein by western blot analysis.

The effects of ustekinumab and CUC on PCNA IE in IQ-induced psoriatic skin lesions.

ISt

IHC-P Image PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis.

IHC

IHC-P Image PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis.

IHC

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PCNA, antibody (Green) at 1:500 dilution.

ICC/IF Image PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.

IHC-P Image PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis.

IHC

IP Image Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 μg of PCBP2 antibody, Western blot analysis was performed using PCBP2 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image PCNA antibody detects PCNA protein at nucleus on mouse liver by immunohistochemical analysis.

IHC

IHC-P Image PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis.

IHC

Lycogen™ reduced cisplatin-induced cell apoptosis.

Combination of bortezomib and citreoviridin inhibited cell cycle progression through different cell cycle regulators.

Thyroid hormone induced proliferation in ovarian cancer cellsOVCAR-3 and SKOV-3 were treated with T3 (10-9 to 10-7 M) or T4 (10-8 to 10-6 M) for 3 days (A), 24 hours (B, upper panel) or 30 min (B, lower panel).

Changes in important mediators of inflammation and fibrosis in the obstructed kidney 7 days after UUO and the effects of Sirt1 activation or inhibition.

Prostate tumors grown from Cav1-silenced PC3 cells were accompanied by a more reactive tumor stroma.

IHC (p)

16 Abbildungen

4 Referenzen

Produktnummer ABIN2854788

583,17 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 7 bis 10 Werktagen

Details

PCNA Antikörper (C-Term)

PCNA Reaktivität: Human WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB17486 unconjugated

Western blot analysis of PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) in Jurkat, Hela, 293 cell line lysates (35 μg/lane).

PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

IHC (p)

Confocal immunofluorescent analysis of PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Confocal immunofluorescent analysis of PCNA Antibody (C-term) (ABIN389344 and ABIN2839453) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Western blot analysis of PCNA (arrow) using rabbit polyclonal PCNA Antibody (C-term) (ABIN389344 and ABIN2839453).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (C-term) (ABIN389344 and ABIN2839453) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

6 Abbildungen

5 Referenzen

Produktnummer ABIN389344

434,77 €

Zzgl. Versandkosten 20,00 € und MwSt

400 μL (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 14 bis 17 Werktagen

Details

Fetuin A Antikörper (AA 200-240) (AbBy Fluor® 594)

AHSG Reaktivität: Human, Maus, Ratte WB Wirt: Kaninchen Polyclonal AbBy Fluor® 594

Immunohistological staining of artery tissues from diabetic subjects showing colocalization of serum proteins and mineral deposits.

IHC (p)

8 Abbildungen

1 reference

Produktnummer ABIN2809553

426,37 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 20 bis 25 Werktagen

Details

GAPDH Antikörper (AA 226-335)

GAPDH Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 3C2 unconjugated

GAPDH monoclonal antibody (M01), clone 3C2 Western Blot analysis of GAPDH expression in A-431 .

GAPDH monoclonal antibody (M01), clone 3C2.

Western Blot analysis of GAPDH expression in transfected 293T cell line by GAPDH monoclonal antibody (M01), clone 3C2.

Detection limit for recombinant GST tagged GAPDH is 0.

ELISA

Immunoprecipitation of GAPDH transfected lysate using anti-GAPDH monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GAPDH MaxPab rabbit polyclonal antibody.

Western Blot detection against Immunogen (37.

9 Abbildungen

9 Referenzen

Produktnummer ABIN560997

651,20 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μg

Lieferung nach: Deutschland

Lieferung in 8 bis 9 Werktagen

Details

GAPDH Antikörper (Internal Region)

Verified GAPDH Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF Wirt: Ziege Polyclonal unconjugated

ABIN185543 (20 µg/mL) staining of PFA-perfused cryosection of Rat Liver.

ABIN185543 (2µg/ml) staining of paraffin embedded Human Liver.

IHC

ABIN185543 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.

ABIN185543 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

11 Abbildungen

6 Referenzen

Produktnummer ABIN185543

488,25 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μg

Lieferung nach: Deutschland

Lieferung in 5 bis 8 Werktagen

Details

beta Actin Antikörper

ACTB Reaktivität: Human, Maus, Huhn, Kaninchen, Schwein, Rind (Kuh), Hamster, Ziege, Fisch WB, ELISA Wirt: Maus Monoclonal 2D1D10 unconjugated

Western Blotting validation image for anti-Actin, beta (ACTB) antibody (ABIN962807)

Lane 1: 20 µg Hela cell lysate Lane 2: 20 µg sp2/0 cell lysate Lane 3: 20 µg goat muscle lysate Lane 4: 20 µg rabbit muscle lysate Lane 5: 20 µg chicken muscle lysate Lane 6: 20 µg CHO cell lysate Lane 7: 20 µg fish muscle lysate Primary antibody: 1 µg/mL Anti-beta-actin Monoclonal Antibody (Mouse) (ABIN396859) Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (ABIN398387, 1: 20,000)

4 Abbildungen

2 Referenzen

1 Validierung

Produktnummer ABIN962807

323,08 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μg (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 10 bis 14 Werktagen

Details

Independently Validated

c-MYC Antikörper (AA 31-80)

MYC Reaktivität: Human, Maus, Ratte WB, IHC, IF, ELISA Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Myc Proto-Oncogene protein (MYC) (AA 31-80) antibody (ABIN1532205)

Immunocytochemistry validation image for anti-Myc Proto-Oncogene protein (MYC) (AA 31-80) antibody (ABIN1532205)

ICC

Western blot analysis of extracts from HeLa cells, treated with Forskolin 40nM 30', using MYC (Ab-62) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MYC (Ab-62) Antibody.

IHC

Immunofluorescence analysis of HeLa cells, using MYC (Ab-62) Antibody.

5 Abbildungen

2 validations

Produktnummer ABIN1532205

402,00 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 6 bis 9 Werktagen

Details

Independently Validated

MFN1 Antikörper (AA 1-234)

MFN1 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Mitofusin 1 (MFN1) (AA 1-234) antibody (ABIN5013951)

IHC

5 Abbildungen

1 Validierung

Produktnummer ABIN5013951

252,00 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 13 bis 17 Werktagen

Details

Independently Validated

JAG1 Antikörper (N-Term)

Rockland 200-401-698 JAG1 Reaktivität: Human, Maus WB, IHC, ELISA, IF, FM Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Jagged 1 (JAG1) (N-Term) antibody (ABIN1043767)

Western blot using Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5).

Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Protein A purified anti-Jagged-1 antibody.

IHC

Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells.

4 Abbildungen

2 Referenzen

1 Validierung

Produktnummer ABIN129524

692,37 €

Zzgl. Versandkosten 20,00 €, Trockeneispauschale 20,00 € sowie MwSt

500 μg (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 5 bis 8 Werktagen

Details

Independently Validated

beta Catenin Antikörper (C-Term)

Rockland 600-401-c68 CATNB Reaktivität: Human, Zebrafisch (Danio rerio) WB, IHC, ELISA Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Catenin, beta (CATNB) (C-Term) antibody (ABIN1043907)

Western Blot of Rabbit anti-Beta Catenin antibody.

Western Blot of Rabbit anti-catenin ß-1 antibody Lane 1: zebrafish embryos injected with myc tagged catenin ß 1 mRNA Lane 2: zebrafish embryos injected with myc tagged catenin ß 2 mRNA Lane 3: zebrafish embryos un-injected Primary antibody: catenin ß-1 antibody at 1:500 overnight at 4°C Secondary antibody: goat anti-rabbit HRP at 1:10,000 for 1 hour at RT Predicted/Observed size: 85.

Immunohistochemistry of Rabbit anti-beta Catenin antibody.

IHC

4 Abbildungen

2 Referenzen

1 Validierung

Produktnummer ABIN1043906

624,85 €

Zzgl. Versandkosten 20,00 €, Trockeneispauschale 20,00 € sowie MwSt

100 μg (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 5 bis 8 Werktagen

Details

Independently Validated

NPM1 Antikörper (C-Term)

NPM1 Reaktivität: Human WB, IF, FACS, IHC (p), IP Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

Immunofluorescence validation image for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

Flow Cytometry validation image for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

FACS

Image no. 2 for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

Image no. 1 for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody (ABIN1737584)

5 Abbildungen

3 validations

Produktnummer ABIN1737584

600,00 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 3 bis 4 Werktagen

Details

Independently Validated

SOD1 Antikörper (AA 2-154)

SOD1 Reaktivität: Human, Pferd WB, IHC, IP, IF/ICC Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody (ABIN3058918)

Immunocytochemistry validation image for anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody (ABIN3058918)

ICC

Western blot analysis of recombinant Horse SOD1.

Western blot analysis of (1) Rat Serum, (2) Bovine Liver Tissue, (3) Bovine Heart Tissue, (4) Bovine Brain Tissue and (5) Bovine Serum.

4 Abbildungen

1 reference

2 validations

Produktnummer ABIN2913314

528,73 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 7 bis 15 Werktagen

Details

Independently Validated

ODF2 Antikörper

ODF2 Reaktivität: Human, Ratte, Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Outer Dense Fiber of Sperm Tails 2 (ODF2) antibody (ABIN2430582)

Immunocytochemistry validation image for anti-Outer Dense Fiber of Sperm Tails 2 (ODF2) antibody (ABIN2430582)

ICC

Western Blotting (WB) image for anti-Outer Dense Fiber of Sperm Tails 2 (ODF2) antibody (ABIN2430583)

Western blot analysis of Human testis tissue and K562 cell, using ODF2 Polyclonal Antibody at dilution of 1:650

4 Abbildungen

2 Referenzen

2 validations

Produktnummer ABIN2430583

449,00 €

Zzgl. Versandkosten 20,00 € und MwSt

200 μL (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 10 bis 13 Werktagen

Details

Independently Validated

Rekombinanter RAD21 Antikörper (N-Term)

RAD21 Reaktivität: Human, Maus WB, IF Wirt: Maus Monoclonal unconjugated Recombinant Antibody

Cleavage Under Targets and Release Using Nuclease validation image for anti-RAD21 Homolog (RAD21) (N-Term) antibody (ABIN6731038)

CUT&RUN

Rad21 antibody (rAb) tested by Western blot.

Rad21 antibody (rAb) tested by Immunofluorescence.

4 Abbildungen

1 reference

1 Validierung

Produktnummer ABIN6731038

561,54 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μg

Lieferung nach: Deutschland

Lieferung in 2 bis 4 Werktagen

Details

Independently Validated

C10orf2 Antikörper (Middle Region)

C10ORF2 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Hund, Kaninchen, Zebrafisch (Danio rerio) WB Wirt: Kaninchen Polyclonal unconjugated