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FACS Antikörper

Fluoreszenz-aktivierte Zellsortierung (FACS) ist eine spezielle Art der Durchflusszytometrie. Sie sortiert ein heterogenes Gemisch biologischer Zellen in zwei oder mehr Behälter, jeweils eine Zelle nach der anderen, auf der Grundlage der spezifischen Lichtstreuungs- und Fluoreszenzeigenschaften jeder Zelle und der an sie gebundenen Antikörper.

Wichtige Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

Alle Flow Cytometry Antibodies

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Vimentin Antikörper

VIM Reaktivität: Säugetier WB, FACS, IHC (p), ICC Wirt: Maus Monoclonal VI-01 unconjugated

Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4).

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-01 ((ABIN94491), diluted 1:400), detected with GAM IgM-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547.

Immunofluorescence staining (rat basophils)

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody.

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01.

Separation of HeLa cells stained using anti-Vimentin (VI-01) purified antibody (concentration in sample 5 μg/mL, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining of methanol permeabilisated cells).

Immunocytochemistry analysis of vimentin in methanol/acetone fixed murine 3T3 cells using mouse monoclonal antibody VI-01.

Anti-Vimentin Purified (VI-01) works in WB application under both reducing and non-reducing conditions.

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4 Referenzen

Produktnummer ABIN94491

CD63 Antikörper (Biotin)

CD63 Reaktivität: Human FACS, IP, IHC (p), ICC Wirt: Maus Monoclonal MEM-259 Biotin

Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue).

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC.

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC) and anti-human CD203c (NP4D6) PE antibody (20 μL reagent / 100 μL of peripheral whole blood).

Separation of CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC).

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3 Referenzen

Produktnummer ABIN1027697

Caspase 1 p20 Antikörper (AA 181-280)

Reaktivität: Human, Maus, Ratte WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro), ICC Wirt: Kaninchen Polyclonal unconjugated

Decreased cAMP exaggerated acute gouty arthritis in P2Y14R-KO rats.

Rat lung lysates probed with Caspase-1 p20 Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Upregulated cAMP suppressed acute gouty arthritis in vivo.

Pyroptosis stress was invalidated once cAMP increased by Forskolin in vitro.

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1 reference

Produktnummer ABIN5675787

STS Antikörper

STS Reaktivität: Human, Maus, Ratte WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Decreased cAMP exaggerated acute gouty arthritis in P2Y14R-KO rats.

Formation of the NLRP3 inflammasome was induced in PBMCs by CSFV infection.

Formation of the NLRP3 inflammasome was induced in PBMCs by CSFV infection.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Upregulated cAMP suppressed acute gouty arthritis in vivo.

Pyroptosis stress was invalidated once cAMP increased by Forskolin in vitro.

9 Abbildungen

2 Referenzen

Produktnummer ABIN2170333

Collagen Type I Antikörper (AA 1051-1150)

COL1 Reaktivität: Human, Maus, Ratte, Kaninchen WB, ELISA, IHC (fro), IHC (p), ICC, IF (cc), IF (p), FACS Wirt: Kaninchen Polyclonal unconjugated

miR155 deletion increases adipose tissue fibrosis under high-fat diet (HFD) conditions in male mice.

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program.

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program.

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

This image was kindly submitted by Kandy Velazquez of USC School of Medicine.

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Produktnummer ABIN2173309

ATG5 Antikörper (AA 101-200)

ATG5 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-APG5L/ATG5 Polyclonal Antibody (ABIN752413) at 1:100 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-APG5L/ATG5 Polyclonal Antibody (ABIN752413) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-APG5L/ATG5 Polyclonal Antibody at 1:100 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-APG5L/ATG5 Polyclonal Antibody ) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-APG5L/ATG5 Polyclonal Antibody (ABIN752413) at 1:100 followed by conjugation to the secondary antibody and DAB staining.

Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti APG5L/ATG5 Polyclonal Antibody, Unconjugated (ABIN752413) at 1:200 in 4 °C.

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Produktnummer ABIN752413

TLR6 Antikörper (AA 301-400)

TLR6 Reaktivität: Human, Maus, Ratte WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-TLR6/CD286 Polyclonal Antibody, Unconjugated (ABIN749573) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Rat splenocytes stained with Anti- TLR6/CD286 Polyclonal Antibody, PE-CY5.

Mouse thymus lysates probed with TLR6 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Rat splenocytes stained with Anti- TLR6/CD286 Polyclonal Antibody, PE-CY5.

A549 cells probed with TLR6 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-TLR6 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Rat spleen lysates probed with TLR6 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Mouse splenocytes probed with TLR6 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

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1 reference

Produktnummer ABIN749573

NGFR Antikörper (AA 301-400)

NGFR Reaktivität: Human, Ratte, Maus WB, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Mouse brain lysates probed with Anti-p75 NGF Receptor Polyclonal Antibody at 1:5000 90min in 37˚C.

Immunohistochemistry (IHC) image for anti-Nerve Growth Factor Receptor (NGFR) (AA 301-400) antibody (ABIN725660)

Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated (ABIN725660) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated (ABIN725660) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated (ABIN725660) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C

HepG2 cells were stained with p75 NGF receptor Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody.

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4 Referenzen

Produktnummer ABIN725660

FLT1 Antikörper (AA 1162-1260)

FLT1 Reaktivität: Human, Ratte, Maus WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Rat lung lysates 30ug, probed at 1:200 overnight in 4˚C.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-VEGFR1/FLT1 Polyclonal Antibody, Unconjugated (ABIN725795) at 1:400 followed by conjugation to the secondary antibody and DAB staining

Mouse spleen cells probed with VEGFR1 Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Image kindly submitted by Victor Blanco as part of the Free Sample Program.

Rat lung lysates 30ug, probed (ABIN725795) at 1:200 overnight in 4 °C.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-VEGFR1/FLT1 Polyclonal Antibody, Unconjugated at 1:400 followed by conjugation to the secondary antibody and DAB staining

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4 Referenzen

Produktnummer ABIN725795

Cytokeratin 7 Antikörper (AA 251-350)

KRT7 Reaktivität: Human, Maus WB, FACS, IHC (p), ELISA, IHC (fro), IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded human lung cancer labeled with Anti-CK7 (Human) Polyclonal Antibody, Unconjugated (ABIN732023) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Rabbit Anti-CK7 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining.

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Produktnummer ABIN732023

SIRT1 Antikörper (AA 101-200)

SIRT1 Reaktivität: Human, Maus, Ratte WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Protein: mouse heart lysate; Primary: rabbit Anti-SIRT1 at 1: 5000; Predicted band size: 58/81 kD Observed band size: 58 kD

Lane 1: mouse liver lysates Lane 2: mouse brain lysates probed with Anti SIRT1 Polyclonal Antibody, Unconjugated (ABIN729853) at 1:200 in 4C.

Formalin-fixed and paraffin embedded human endometrial carcinoma labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (ABIN729853) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (ABIN729853) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Hela cell lysates probed with Anti-SIRT1 Polyclonal Antibody, Unconjugated at 1:3000 90min in 37˚C.

MCF-7 cells probed with SIRT1 Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

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5 Referenzen

Produktnummer ABIN729853

FASL Antikörper (AA 196-281)

FASL Reaktivität: Human, Maus, Ratte, Schwein WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Effects of different p,p'-DDE concentrations on the FasL and caspase-3 and -8 mRNA in rat Sertoli cells by RT-PCR (a)-(c).

Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-FasL Polyclonal Antibody, Unconjugated (ABIN726410) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-FasL Polyclonal Antibody, Unconjugated (ABIN726410) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-FasL Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-FasL Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Jurkat lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated at 1:1000 dilution and 4˚C overnight incubation.

Bone lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

(a) Effect of NAC on p,p'-DDE-induced FasL protein.

Effects of BDE47 on the production of intracellular ROS and the expression of apoptosis-related proteins in GC-1 cells.

Effect of BDE47 on daily sperm production and germ cell apoptosis in seminiferous tubules.

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Produktnummer ABIN726410

ERK1/2 Antikörper (AA 301-358)

MAPK1/3 Reaktivität: Human, Maus, Ratte, Schwein WB, ELISA, IHC (p), IF (cc), FACS, IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Mouse placenta lysates probed with ERK1 + ERK2 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated (ABIN723725) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rat hippocampus labeled with Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated (ABIN723725) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated (ABIN723725) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C.

L1 rat brain, L2 rat heart lysates probed (ABIN723725) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated (ABIN723725) at 1:200 followed by conjugation to the secondary antibody and DAB staining

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4 Referenzen

Produktnummer ABIN723725

CREB1 Antikörper (pSer133)

CREB1 Reaktivität: Human, Maus, Ratte, Schwein WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

U251 lysates probed with CREB-1(Ser133) Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody (ABIN723980) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse hippocampus labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody

Rat RSC96 cells probed with CREB1 (Ser133) Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated (ABIN723980) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Mouse spleen lysate probed with Anti-CREB-1(Ser133) Polyclonal Antibody at 1:5000 90min in 37˚C.

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1 reference

Produktnummer ABIN723980

ERK1 Antikörper (AA 101-200)

MAPK3 Reaktivität: Human, Maus, Ratte, Huhn WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti ERK1/MAPK3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti ERK1/MAPK3 Polyclonal Antibody, Unconjugated (ABIN736476) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat colon labeled with Rabbit Anti ERK1/MAPK3 Polyclonal Antibody, Unconjugated (ABIN736476) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Mouse splenocytes probed with Rabbit Anti-ERK1 Polyclonal Antibody, Unconjugated .

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

7 Abbildungen

1 reference

Produktnummer ABIN736476

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