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Glucose Oxidase Antikörper

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Glucose Oxidase in ELISA und WB. Er zeigt eine Reaktivität gegenüber Aspergillus niger.
Rockland
Produktnummer ABIN99703
Hersteller Produkt- Nr.: 100-1137

Kurzübersicht für Glucose Oxidase Antikörper (ABIN99703)

Target

Glucose Oxidase (GOD)

Reaktivität

  • 16
  • 15
  • 3
Aspergillus niger

Wirt

  • 25
  • 4
  • 3
  • 2
Ziege

Klonalität

  • 30
  • 4
Polyklonal

Konjugat

  • 14
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Glucose Oxidase Antikörper ist unkonjugiert

Applikation

  • 31
  • 20
  • 13
  • 13
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
ELISA, Western Blotting (WB)
  • Hersteller Produkt- Nr.

    100-1137

    Hersteller

    Rockland

    Verwendungszweck

    Glucose Oxidase Antibody

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glucose Oxidase [Aspergillus niger].

    Produktmerkmale

    Synonyms: goat anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

    Aufreinigung

    Anti-glucose oxidase antibody was prepared from monospecific antiserum by a delipidation and defibrination.

    Immunogen

    Immunogen: Glucose Oxidase [Aspergillus niger]

    Immunogen Type: Native Protein

  • Applikationshinweise

    Application Note: Anti-glucose oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Optimal concentration should be determined by the researcher.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    90 mg/mL

    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: None

    Konservierungsmittel

    Without preservative

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    Glucose Oxidase (GOD)

    Andere Bezeichnung

    Glucose Oxidase

    Hintergrund

    Background: Glucose oxidase antibody is specific to glucose oxidase enzyme. Glucose oxidase is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. Glucose oxidase is commonly used for the determination of free glucose in body fluids (diagnostics), and in the food industry. It also has many applications in biotechnologies, for example enzyme assays for biochemistry including biosensors in nanotechnologies. Glucose oxidase is often extracted from Aspergillus niger.

    UniProt

    P13006
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