Fructosyl Amino Acid Oxidase Antikörper

Produktnummer ABIN99448

Hersteller Produkt- Nr.: 200-601-236-0100

Der Schaf Polyklonal anti-Fructosyl Amino Acid Oxidase Antikörper wird Verwendung zum Nachweis von Fructosyl Amino Acid Oxidase in proben von Corynebacterium. Er wurde validiert für WB, IP und ELISA.

Kurzübersicht für Fructosyl Amino Acid Oxidase Antikörper (ABIN99448)

Target: Fructosyl Amino Acid Oxidase (socD)

Reaktivität: Corynebacterium

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Fructosyl Amino Acid Oxidase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Produktdetails anti-Fructosyl Amino Acid Oxidase Antikörper

Hersteller Produkt- Nr.: 200-601-236-0100

Hersteller: Rockland

Verwendungszweck: Fructosyl-Amino Acid Oxidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.

Produktmerkmale: Synonyms: sheep anti-Fructosyl-Amino Acid Oxidase Antibody, Fructosyl-amino acid oxidase

Aufreinigung: Anti-FRUCTOSYL-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Fructosyl-amino acid oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Fructosyl Amino Acid Oxidase

Target: Fructosyl Amino Acid Oxidase (socD)

Andere Bezeichnung: Fructosyl-Amino Acid Oxidase

Hintergrund: Background: Fructosyl amino acid oxidase is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide. Anti-Fructosyl-amino acid oxidase can be useful for researchers interested in diabetes research.

UniProt: Q8RIU8