Fructosyl Amino Acid Oxidase Antikörper

Produktnummer ABIN99448

Hersteller Produkt- Nr.: 200-601-236-0100

Der Schaf Polyklonal Anti-Fructosyl Amino Acid Oxidase-Antikörper wurde für WB, IP und ELISA validiert. Er ist geeignet, Fructosyl Amino Acid Oxidase in Proben von Corynebacterium zu detektieren.

Kurzübersicht für Fructosyl Amino Acid Oxidase Antikörper (ABIN99448)

Target: Fructosyl Amino Acid Oxidase (socD)

Reaktivität: Corynebacterium

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Fructosyl Amino Acid Oxidase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Produktdetails anti-Fructosyl Amino Acid Oxidase Antikörper

Hersteller Produkt- Nr.: 200-601-236-0100

Hersteller: Rockland

Verwendungszweck: Fructosyl-Amino Acid Oxidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.

Produktmerkmale: Synonyms: sheep anti-Fructosyl-Amino Acid Oxidase Antibody, Fructosyl-amino acid oxidase

Aufreinigung: Anti-FRUCTOSYL-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Fructosyl-amino acid oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Fructosyl Amino Acid Oxidase

Target: Fructosyl Amino Acid Oxidase (socD)

Andere Bezeichnung: Fructosyl-Amino Acid Oxidase

Hintergrund: Background: Fructosyl amino acid oxidase is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide. Anti-Fructosyl-amino acid oxidase can be useful for researchers interested in diabetes research.

UniProt: Q8RIU8