CES3 Antikörper

Produktnummer ABIN98993

Hersteller Produkt- Nr.: 200-4169-0100

Der Kaninchen Polyklonal Anti-CES3-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, CES3 in Proben von Schwein zu detektieren.

Kurzübersicht für CES3 Antikörper (ABIN98993)

Target: CES3 (Carboxylesterase 3 (CES3))

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CES3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CES3 Antikörper

Hersteller Produkt- Nr.: 200-4169-0100

Hersteller: Rockland

Verwendungszweck: Esterase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].

Produktmerkmale: Synonyms: rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase

Aufreinigung: Anti-Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Esterase [Porcine Liver]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Esterase is suitable for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CES3

Target: CES3 (Carboxylesterase 3 (CES3))

Andere Bezeichnung: CES3

Hintergrund: Background: Esterase is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It is active towards triacylglycerides containing short-chain fatty acids from C2 to C6, and 13-monoacylglycerols containing fatty acids from C2 to C12, and inactive on long-chain triacylglycerols and diacylglycerol. it hydrolyzes aromatic and alkyl esters and vitamin A acetate. The hydrolysis rate depends upon the amino acid promoiety and the esterification site of the prodrug. Aromatic promoieties are favored, highest rates are observed with phenylalanyl progdrugs, hydrolysis of valyl and isoleucyl prodrugs is less efficient. With floxuridine prodrugs, activity is higher on 5' monoesters than on 3' monoesters, while with gemcitabine prodrugs, activity is higher on 3' monoesters than on 5' monoesters.

Gen-ID: 100626873, 343791015

UniProt: Q29550

Pathways: Monocarboxylic Acid Catabolic Process