HDAC1 Antikörper (AA 385-396)

Produktnummer ABIN987284

Der Ziege Polyklonal Anti-HDAC1-Antikörper wurde für WB, ELISA und ChIP validiert. Er ist geeignet, HDAC1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für HDAC1 Antikörper (AA 385-396) (ABIN987284)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HDAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Güteklasse: Verified

Produktdetails anti-HDAC1 Antikörper

Bindungsspezifität: AA 385-396

Verwendungszweck: Hdac1 (aa385-396)

Sequenz: PEDAIPEESG DE

Spezifität: This antibody may cross-react to HDAC2

Kreuzreaktivität: Rind (Kuh), Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PEDAIPEESGDE, from the internal region of the protein sequence according to NP_032254.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 55 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 55.1 kDa according to Human NP_032254.1 and Mouse NP_032254.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Kommentare:

Chromatin Immunoprecipitation: A 4-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB11428 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Andere Bezeichnung: Hdac1

Hintergrund: HDAC1, histone deacetylase 1, RP23-209C6.7, HD1, Hdac1-ps, MommeD5, RPD3, MGC102534, MGC118085, OTTMUSP00000010171

Gen-ID: 3065, 433759, 84576

NCBI Accession: NP_032254

Pathways: Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis