Cyanine Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN97919

Hersteller Produkt- Nr.: 600-605-209

Der Schaf Polyklonal Anti-Cyanine-Antikörper wurde für ELISA, IHC, WB und DB validiert. Er ist geeignet, Cyanine in Proben von zu detektieren.

Kurzübersicht für Cyanine Antikörper (Alkaline Phosphatase (AP)) (ABIN97919)

Target: Cyanine

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Cyanine Antikörper ist konjugiert mit Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Produktdetails anti-Cyanine Antikörper (Alkaline Phosphatase (AP))

Hersteller Produkt- Nr.: 600-605-209

Hersteller: Rockland

Verwendungszweck: Cyanine Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum and cyanine conjugated IgG.

Produktmerkmale: Synonyms: sheep anti-Cyanine Antibody alkaline phosphatase Conjugation, alk phos conjugated sheep anti-Cyanine Antibody

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen:

Immunogen: A mixture of Cy3 and Cy5 conjugated KLH

Immunogen Type: Other

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Cyanine Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and dot blot. This product has been assayed against 1.0 μg of Cyanine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:700 to 1:2,800 of the reconstitution concentration is suggested for this product. 

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.4 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Details zu Cyanine

Target: Cyanine

Substanzklasse: Chemical

Hintergrund: Background: Cyanine is a synthetic family of dyes that can cover the spectrum from infrared to ultraviolent light. Cyanine is useful in protein and nucleic acid labeling. This Anti-Cyanine Antibody is conjugated to Alkaline Phosphatase. Anti-Cyanine (sheep) antibody is ideal for investigators in Cell Biology, Immunology, and Microbiology research.