Cholesterol Oxidase Antikörper (Biotin)

Produktnummer ABIN97251

Hersteller Produkt- Nr.: 200-106-213-0100

Der Ziege Polyklonal Anti-Cholesterol Oxidase-Antikörper wurde für ELISA, WB, IP und DB validiert. Er ist geeignet, Cholesterol Oxidase in Proben von Streptomyces zu detektieren.

Kurzübersicht für Cholesterol Oxidase Antikörper (Biotin) (ABIN97251)

Target: Cholesterol Oxidase (choS)

Reaktivität: Streptomyces

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Cholesterol Oxidase Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Produktdetails anti-Cholesterol Oxidase Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-106-213-0100

Hersteller: Rockland

Verwendungszweck: Cholesterol Oxidase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism].

Produktmerkmale: Synonyms: goat anti-Cholesterol Oxidase Antibody biotin Conjugation, biotin Conjugated goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

Aufreinigung: Anti-Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Cholesterol Oxidase [Streptomyces]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Cholesterol Oxidase has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 μg of Cholesterol Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Cholesterol Oxidase

Target: Cholesterol Oxidase (choS)

Andere Bezeichnung: choA

Hintergrund: Background: Anti-Cholesterol Oxidase antibody is a member of the GMC oxidoreductase family. Cholesterol Oxidase acts as a catalyst in the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of new product. Anti-Cholesterol Oxidase is ideal for investigators interested in Metabolism, Signal Transduction, or Cardiovascular research.

UniProt: P12676