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Vimentin Antikörper (AA 2-466)

Name: Vimentin Antikörper (AA 2-466)
Brand: antibodies-online
SKU: ABIN969454
Price: 658.71 EUR
Availability: InStock

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Vimentin in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human und Affe und wurde in 2+ Publikationen erwähnt.

Western blot analysis using Vimentin mouse mAb against Hela (1), COS (2), HEK293 (3) and U20S (4) cell lysate. (Vimentin Antikörper (AA 2-466))

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining. (Vimentin Antikörper (AA 2-466))

IHC

2 Abbildungen

(2 Referenzen)

Produktnummer ABIN969454

Kurzübersicht für Vimentin Antikörper (AA 2-466) (ABIN969454)

Target

Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)

Reaktivität

Human, Affe

Wirt

Maus

Klonalität

Monoklonal

Konjugat

Dieser Vimentin Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Klon

4F2E9

Discover our top product VIM Primärantikörper

Bindungsspezifität
33

32

17

16

12

10

9

7

5

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-466

Verwendungszweck

Vimentin Antibody

Aufreinigung

Ascitic fluid

Immunogen

Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli.

Isotyp

IgG1
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Affe WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Huhn WB, IHC (p), ICC, IP Wirt: Maus Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Säugetier WB, FACS, IHC (p), ICC Wirt: Maus Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Maus Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, IF, IP Wirt: Kaninchen Monoclonal unconjugated

Applikationshinweise

ELISA: 1/10000

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." in: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, (2007) (PubMed).

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." in: Cancer research, Vol. 63, Issue 10, pp. 2658-64, (2003) (PubMed).
Target

Vimentin (VIM)

Andere Bezeichnung

Vimentin

Hintergrund

Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Molekulargewicht

54 kDa

Gen-ID

7431

UniProt

P08670

Pathways

Caspase Kaskade in der Apoptose