OCT4 Antikörper

Produktnummer ABIN969329

Der Maus Monoklonal Anti-OCT4-Antikörper wurde für WB, ELISA, ICC und FACS validiert. Er ist geeignet, OCT4 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für OCT4 Antikörper (ABIN969329)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 7E7

Produktdetails anti-OCT4 Antikörper

Verwendungszweck: 4-Oct Antibody

Aufreinigung: Ascitic fluid

Immunogen: Synthesized peptide derived from internal of human Oct4.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-OCT4 Antikörper (ABIN969329)

Wang, Dai: "Concise review: isoforms of OCT4 contribute to the confusing diversity in stem cell biology." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 5, pp. 885-93, (2010) (PubMed).

Stefanovic, Pucéat: "[The dual role of OCT4]." in: Médecine sciences : M/S, Vol. 26, Issue 4, pp. 411-6, (2010) (PubMed).

Rose, Behm, Drgon, Johnson, Uhl: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, (2010) (PubMed).

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: 4-Oct

Hintergrund: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. (provided by RefSeq).Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Molekulargewicht: 45 kDa

UniProt: Q01860

Pathways: Stem Cell Maintenance