TRIP10 Antikörper (AA 411-501)

Produktnummer ABIN968876

Der Maus Monoklonal Anti-TRIP10-Antikörper wurde für WB und IF validiert. Er ist geeignet, TRIP10 in Proben von Human, Maus, Ratte und Hund zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für TRIP10 Antikörper (AA 411-501) (ABIN968876)

Target: TRIP10 (Thyroid Hormone Receptor Interactor 10 (TRIP10))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TRIP10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 21-CIP4

Produktdetails anti-TRIP10 Antikörper

Bindungsspezifität: AA 411-501

Kreuzreaktivität: Hund, Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CIP4 aa. 411-501

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-TRIP10 Antikörper (ABIN968876)

Richnau, Aspenström: "Rich, a rho GTPase-activating protein domain-containing protein involved in signaling by Cdc42 and Rac1." in: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 35060-70, (2001) (PubMed).

Tian, Nelson, Stewart: "Cdc42-interacting protein 4 mediates binding of the Wiskott-Aldrich syndrome protein to microtubules." in: The Journal of biological chemistry, Vol. 275, Issue 11, pp. 7854-61, (2000) (PubMed).

Aspenström: "A Cdc42 target protein with homology to the non-kinase domain of FER has a potential role in regulating the actin cytoskeleton." in: Current biology : CB, Vol. 7, Issue 7, pp. 479-87, (1997) (PubMed).

Details zu TRIP10

Target: TRIP10 (Thyroid Hormone Receptor Interactor 10 (TRIP10))

Andere Bezeichnung: Cip4

Hintergrund: Rho family members are small GTP binding proteins that serve as molecular switches for a number of biological processes. They cycle between active GTP-bound and inactive GDP-bound states. CDC42 is a Rho family protein that was identified in membranes of human platelets and placenta. It is the homologue of CDC42Sc, which regulates initiation of bud-site assembly in Saccharomyces cerevisiae. Similarly, CDC42 regulates the function of the mammalian actin cytoskeleton, allowing for efficient cytokinesis and cell morphogenesis. CDC42-interacting protein 4 (CIP4) was identified in a yeast-two hybrid screen for proteins that bind CDC42. Another variant of CIP4, CIP4/2, was identified that contains an extra 56 amino acids and has 71% identity with CIP4 (or CIP4/1). CIP4 contains a C-terminal SH3 domain and an N-terminal domain that is homologous to non-catalyitic motifs in the tyrosine kinase Fer. The mRNA expression of CIP4 is highest in skeletal muscle, heart, and placenta. Overexpression of CIP4 in Swiss 3T3 cells reduces the amount of stress fibers and leads to clustering of CIP4 to foci at the dorsal side of the cells. In addition, CIP4 binds the Rho-GTPase activating protein RICH and the cytoskeletal protein WASP. Coexpression of CIP4 and WASP in Cos-7 cells leads to WASP association with microtubules. Thus, CIP4 is involved in various protein-protein interactions associated with cytoskeletal dynamics. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 80 kDa