HNF1B Antikörper (AA 1-101)

Produktnummer ABIN968855

Der Maus Monoklonal Anti-HNF1B-Antikörper wurde für WB und IF validiert. Er ist geeignet, HNF1B in Proben von Human, Ratte, Maus und Hund zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für HNF1B Antikörper (AA 1-101) (ABIN968855)

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HNF1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 25-HNF

Produktdetails anti-HNF1B Antikörper

Bindungsspezifität: AA 1-101

Kreuzreaktivität: Ratte (Rattus), Human, Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse HNF-1beta aa. 1-101

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968547, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-HNF1B Antikörper (ABIN968855)

Soubt, Marksitzer, Menoud, Nagamine: "Role of tissue-specific transcription factor LFB3 in a cyclic AMP-responsive enhancer of the urokinase-type plasminogen activator gene in LLC-PK1 cells." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4698-706, (1998) (PubMed).

Ringeisen, Rey-Campos, Yaniv: "The transactivation potential of variant hepatocyte nuclear factor 1 is modified by alternative splicing." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25706-11, (1994) (PubMed).

De Simone, De Magistris, Lazzaro, Gerstner, Monaci, Nicosia, Cortese: "LFB3, a heterodimer-forming homeoprotein of the LFB1 family, is expressed in specialized epithelia." in: The EMBO journal, Vol. 10, Issue 6, pp. 1435-43, (1991) (PubMed).

Details zu HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Andere Bezeichnung: HNF-1beta

Hintergrund: HNF's (Hepatocyte Nuclear Factor) are POU-homeodomain-containing liver enriched transcription factors that include HNF1-alpha, HNF1-beta, HNF-3alpha, HNF-3beta, HNF-4 and HNF-6. HNF-1 is expressed in liver, kidney, and intestine, while HNF-1beta is expressed highest in kidney with lower levels in intestine, lung, and liver. Both HNF-1 and HNF-1beta have short N-terminal alpha-helical segments that facilitate homodimerization (A-domain), a region related to POU-specific A-box (B-domain), and a homeodomain (C-domain). The HNF-1 and HNF-1beta homo- and hetero-dimers transactivate genes required for development of various tissues. Both transcription factors can bind the consensus HNF1 inverted palindromic sequence GTTAATNATTAAC. HNF-1 represses its own transcription and data suggests that there is coordinate regulation between HNF-1 and HNF-4. Although HNF-4 induces HNF-1 transcription, HNF-1 downregulates HNF-4 activity by binding to the AF2 region in the activation domain of HNF-4. Thus, HNF transcription factors may be involved in various aspects of the regulation of gene transcription throughout development.
Synonyms: Hepatocyte Nuclear Factor-1beta

Molekulargewicht: 51 kDa

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation