LCK Antikörper (pTyr505)

Produktnummer ABIN968843

Produktdetails anti-LCK Antikörper

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Bindungsspezifität: pTyr505

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LCK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Kreuzreaktivität: Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Lck (pY505)

Klon: 4-Lck

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967742, ABIN968656

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-LCK Antikörper (ABIN968843)

Wang, Lemay, Tsai, Veillette: "SH2 domain-mediated interaction of inhibitory protein tyrosine kinase Csk with protein tyrosine phosphatase-HSCF." in: Molecular and cellular biology, Vol. 21, Issue 4, pp. 1077-88, (2001) (PubMed).

Hardwick, Sefton: "The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505." in: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25429-32, (1997) (PubMed).

Lee-Fruman, Collins, Burakoff: "Role of the Lck Src homology 2 and 3 domains in protein tyrosine phosphorylation." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25003-10, (1996) (PubMed).

Details zu LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Andere Bezeichnung: Lck (LCK Produkte)

Synonyme: zgc:136695 antikoerper, LCK antikoerper, Hck-3 antikoerper, Lsk antikoerper, Lskt antikoerper, p56 antikoerper, p56Lck antikoerper, LSK antikoerper, YT16 antikoerper, p56lck antikoerper, pp58lck antikoerper, P56LCK antikoerper, tkl antikoerper, Lck1 antikoerper, Lcktkr antikoerper, LCK proto-oncogene, Src family tyrosine kinase antikoerper, lymphocyte protein tyrosine kinase antikoerper, lck antikoerper, LCK antikoerper, Lck antikoerper

Hintergrund: Protein tyrosine phosphorylation is an essential step in the signal transduction cascade leading to T cell antigen receptor (TCR) activation. Lck is a protein kinase and a member of the src family of cytoplasmic protein-tyrosine kinases (PTKs). Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. CD4 and CD8 bind to surface MHC class II and class I molecules, respectively. Lck is regulated by both kinases and phosphatases. Autophosphorylation at Y394 leads to conformational changes in the catalytic domain, which induces kinase activity. Repression of Lck occurs via phosphorylation at Y505, located near the carboxy-terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs. Upon phosphorylation at this site, Lck associates with the SH2 domain in the amino-terminus, thus keeping the protein biologically inactive. Lck activity and regulation is critical for activation and development of T cells.

Molekulargewicht: 56 kDa

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling