Tyrosine Hydroxylase Antikörper (AA 18-133)

Produktnummer ABIN968808

Dieses Anti-Tyrosine Hydroxylase-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Tyrosine Hydroxylase in WB und IF. Geeignet für Ratte und Maus. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für Tyrosine Hydroxylase Antikörper (AA 18-133) (ABIN968808)

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Tyrosine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 45-Tyrosine Hydroxylase

Produktdetails anti-Tyrosine Hydroxylase Antikörper

Bindungsspezifität: AA 18-133

Kreuzreaktivität: Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Tyrosine Hydroxylase aa. 18-133

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968545

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Tyrosine Hydroxylase Antikörper (ABIN968808)

Blanchard-Fillion, Souza, Friel, Jiang, Vrana, Sharov, Barrón, Schöneich, Quijano, Alvarez, Radi, Przedborski, Fernando, Horwitz, Ischiropoulos: "Nitration and inactivation of tyrosine hydroxylase by peroxynitrite." in: The Journal of biological chemistry, Vol. 276, Issue 49, pp. 46017-23, (2001) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: Tyrosine Hydroxylase

Hintergrund: Dopamine and its metabolic products norepinephrine and epinephrine are catecholamine neurotransmitters whose function is essential for the execution of normal neural processes in the CNS and PNS. Tyrosine hydroxylase (TH) is a non-heme iron, tetrahydrobiopterin-dependent enzyme that catalzyes the conversion of tyrosine to L-dihydroxyphenylalanine (L-DOPA). This is the rate-limiting step in the biosynthesis of catecholamines. Both the development of Parkinson’s disease and other neurodegenerative diseases result from loss of the ability to synthesize catecholamines. Decreases in the activity of TH have been implicated in these diseases. Nitration of TH at Tyrosine 423 has been associated with temporary loss of enzymatic activity, and TH nitration occurs in response to the Parkinsonian toxin MPTP, and following exposure to peroxynitrite. These findings implicate nitration as a potential mode of down-regulation of TH activity during neurodegenerative disease. Thus, TH is an essential enzyme for catecholamine synthesis, which is required for normal neuronal function.

Molekulargewicht: 58 kDa

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour