Nicastrin Antikörper (AA 168-289)

Produktnummer ABIN968805

Dieses Anti-Nicastrin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Nicastrin in WB und IF. Geeignet für Human, Maus, Ratte, Huhn und Hund. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für Nicastrin Antikörper (AA 168-289) (ABIN968805)

Target: Nicastrin (NCSTN)

Reaktivität: Human, Maus, Ratte, Huhn, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nicastrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 35-Nicastrin

Produktdetails anti-Nicastrin Antikörper

Bindungsspezifität: AA 168-289

Kreuzreaktivität: Huhn, Hund, Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Nicastrin aa. 168-289

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968553

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Nicastrin Antikörper (ABIN968805)

Chen, Yu, Arawaka, Nishimura, Kawarai, Yu, Tandon, Supala, Song, Rogaeva, Milman, Sato, Yu, Janus, Lee, Song, Zhang, Fraser, St George-Hyslop: "Nicastrin binds to membrane-tethered Notch." in: Nature cell biology, Vol. 3, Issue 8, pp. 751-4, (2001) (PubMed).

Yu, Nishimura, Arawaka, Levitan, Zhang, Tandon, Song, Rogaeva, Chen, Kawarai, Supala, Levesque, Yu, Yang, Holmes, Milman, Liang, Zhang, Xu, Sato, Rogaev, Smith, Janus, Zhang, Aebersold, Farrer, Sorbi et al.: "Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing. ..." in: Nature, Vol. 407, Issue 6800, pp. 48-54, (2000) (PubMed).

Details zu Nicastrin

Target: Nicastrin (NCSTN)

Andere Bezeichnung: Nicastrin

Hintergrund: Amyloid precursor protein (APP) gene encodes multiple APPs ranging from 695 to 770 amino acids. The unprocessed form of APP is a putative cell surface receptor that possesses neurite-promoting activity, and is involved in synaptic vesicle recycling. Processing of APP by sequential enzymatic activity of beta- and gamma-secretase, and the presenilin proteins PS1 and PS2, produces APP fragments that may have unique functions. beta-secretase activity cleaves the extracellular portion of APP leading to asecreted APP form, while gamma-secretase activity produces beta-Amyloid peptide (39-43 amino acids). The Abeta peptide produces abnormal plaques in the cerebral cortex and blood vesselwalls during Alzheimer's disease. Nicastrin is a PS1 associated transmembrane protein,that contains N-terminal glycosylation and myristoylation sites. Nicastrin can bind full length APP and the fragments produced by gamma-secretase. In C. elegans, suppression of nicastrin expression produces phenotypes that mimic those produced when notch signaling proteins are suppressed. In Drosophila, deficiencies in nicastrin prevent cleavage of the intracellular portion of Notch. Thus, nicastrin may be a functional component of presenilins and gamma-secretase complexes, which process Notch and APP transmembrane receptors.

Molekulargewicht: 110 kDa

Pathways: Notch Signalweg, Neurotrophin Signalübertragung