ARHGAP4 Antikörper (AA 843-955)

Produktnummer ABIN968790

Dieses Anti-ARHGAP4-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ARHGAP4 in WB und IF. Geeignet für Maus und Ratte. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für ARHGAP4 Antikörper (AA 843-955) (ABIN968790)

Target: ARHGAP4 (rho GTPase Activating Protein 4 (ARHGAP4))

Reaktivität: Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ARHGAP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 15-p115

Produktdetails anti-ARHGAP4 Antikörper

Bindungsspezifität: AA 843-955

Kreuzreaktivität: Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat p115 aa. 843-955

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-ARHGAP4 Antikörper (ABIN968790)

Mary, Charrasse, Meriane, Comunale, Travo, Blangy, Gauthier-Rouvière: "Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 285-301, (2002) (PubMed).

Barroso, Nelson, Sztul: "Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 2, pp. 527-31, (1995) (PubMed).

Waters, Clary, Rothman: "A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack." in: The Journal of cell biology, Vol. 118, Issue 5, pp. 1015-26, (1992) (PubMed).

Details zu ARHGAP4

Target: ARHGAP4 (rho GTPase Activating Protein 4 (ARHGAP4))

Andere Bezeichnung: p115

Hintergrund: Maturation and post translational modification of proteins occurs after their biosynthesis at the endoplasmic reticulum and their transport through the Golgi apparatus. The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus. p115 is a 959 amino acid protein located at the Golgi apparatus that, with the NEM-sensitive fusion protein and the soluble NSF attachment protein (SNAP), is required for vesicle transport from the cis-compartment to the medial-compartment. p115 protein is related to the yeast Uso1p essential for the vesicular transport from the endoplasmic reticulum to the Golgi. Native p115 appears to be a homo-oligomer, with two globular heads and a tail that resemble the overall structure of myosin. p115 is extracted from the Golgi apparatus with high salt or high pH, indicative of a membrane associated protein. p115 interacts with the golgi matrix protein GM130 but this interaction is disrupted by the Golgi fragmentation during mitosis and the phosphorylation of GM130. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 115 kDa

Pathways: Neurotrophin Signalübertragung, Regulation of Cell Size