Cofilin Antikörper (AA 3-98)

Produktnummer ABIN968755

Produktdetails anti-Cofilin Antikörper

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Bindungsspezifität: AA 3-98

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cofilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Human, Hund, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Cofilin aa. 3-98

Klon: 32-Cofilin

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968540, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Cofilin Antikörper (ABIN968755)

Chan, Bailly, Zebda, Segall, Condeelis: "Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion." in: The Journal of cell biology, Vol. 148, Issue 3, pp. 531-42, (2000) (PubMed).

Moriyama, Yahara: "Two activities of cofilin, severing and accelerating directional depolymerization of actin filaments, are affected differentially by mutations around the actin-binding helix." in: The EMBO journal, Vol. 18, Issue 23, pp. 6752-61, (2000) (PubMed).

Moriyama, Matsumoto, Nishida, Sakai, Yahara: "Nucleotide sequence of mouse cofilin cDNA." in: Nucleic acids research, Vol. 18, Issue 10, pp. 3053, (1990) (PubMed).

Details zu Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Andere Bezeichnung: Cofilin (CFL1 Produkte)

Synonyme: cfl1 antikoerper, xac1 antikoerper, xac2 antikoerper, cfl antikoerper, cb86 antikoerper, sb:cb86 antikoerper, zgc:56501 antikoerper, wu:fb18a04 antikoerper, wu:fk77b03 antikoerper, 12/8 antikoerper, ADF antikoerper, ADF/cofilin antikoerper, Adf antikoerper, CG4254 antikoerper, Cadf antikoerper, D-cof antikoerper, D61 antikoerper, Dmel\\CG4254 antikoerper, T8 antikoerper, Tsr antikoerper, cofilin antikoerper, cofilin/ADF antikoerper, cofilin/actin depolymerizing factor homolog antikoerper, l(2)03101 antikoerper, l(2)k03101 antikoerper, l(2)k05633 antikoerper, l(2)k13409 antikoerper, ntf antikoerper, CFL antikoerper, AA959946 antikoerper, Cof antikoerper, CFL1 antikoerper, cofilin 1 (non-muscle) L homeolog antikoerper, cofilin 1 (non-muscle) antikoerper, cofilin 1 (non-muscle), like antikoerper, cofilin 1 antikoerper, twinstar antikoerper, cofilin antikoerper, cofilin 1, non-muscle antikoerper, Contrafreeloading antikoerper, cfl1.L antikoerper, cfl1 antikoerper, cfl1l antikoerper, CFL1 antikoerper, tsr antikoerper, COF1 antikoerper, TSTA_042790 antikoerper, Cfl1 antikoerper, CFL antikoerper

Hintergrund: Cell motility is a basic cellular behavior involved in embryogenesis, neurite outgrowth, wound healing, inflammation, and cancer metastasis. Filamentous actin is an essential component of cell protrusions involved in cell motility. The protrusion formation is regulated by actin polymerization and depolymerization. Cofilin is a ubiquitously expressed G- and F-actin binding protein that contains a nuclear localization signal, a C-terminal hexapeptide sequence that is identical to tropomyosin, and other regions that are homologous to actin binding proteins. In vitro, cofilin has actin severing activity and induces an increased off-rate from the pointed end of actin filaments. These activities can increase the rate of actin polymerization and depolymerization, as well as increase the number of barbed ends available for polymerization. Stimulation of MTLn3 cells with EGF leads to an increase in cofilin at the leading edge of laemellipodia, which correlates with an increase in the number of barbed ends at the leading edge. Cofilin function blocking antibodies inhibit both the appearance of these barbed ends and lamellipodial protrusion. Thus, cofilin is an actin binding protein that regulates actin filament formation during cell motility.

Molekulargewicht: 19 kDa

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events