Coilin Antikörper (AA 226-332)

Produktnummer ABIN968730

Dieses Anti-Coilin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Coilin in WB und IF. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für Coilin Antikörper (AA 226-332) (ABIN968730)

Target: Coilin (COIL)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Coilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 56-Coilin

Produktdetails anti-Coilin Antikörper

Bindungsspezifität: AA 226-332

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Coilin aa. 226-332

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968586, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Coilin Antikörper (ABIN968730)

Bellini, Gall: "Coilin shuttles between the nucleus and cytoplasm in Xenopus oocytes." in: Molecular biology of the cell, Vol. 10, Issue 10, pp. 3425-34, (1999) (PubMed).

Andrade, Chan, Raska, Peebles, Roos, Tan: "Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin." in: The Journal of experimental medicine, Vol. 173, Issue 6, pp. 1407-19, (1991) (PubMed).

Details zu Coilin

Target: Coilin (COIL)

Andere Bezeichnung: Coilin

Hintergrund: Ramon Cajal described small silver-staining organelles in the nuclei of pyramidal neurons, which have been referred to as cajal bodies or coiled bodies. An 80 kDa protein called p80 coilin was identified through its co-localization to coiled bodies. Coilin is a molecular marker of coiled bodies, but can also be found in the nucleoplasm, and may shuttle back and forth between the cytoplasm and nucleoplasm. Studies of GFP-coilin show that coiled bodies can move within the nucleoplasm to and from nucleoli. Other proteins that have been co-localized with coilin to the coiled bodies include cell cycle proteins, snRNPs, U3 snRNA, U7 snRNA, and several nucleolar proteins. Coiled bodies may recruit U7 snRNP and the stem-loop-binding protein to the chromosomal sites of histone gene transcription. Possibly, coilin is important for the formation of coiled bodies that act as sites for preassembly of transcriptosomes, which facilitate gene transcription and RNA processing.

Molekulargewicht: 80 kDa

Pathways: Ribonucleoprotein Complex Subunit Organization