Synaptophysin Antikörper (AA 205-306)

Produktnummer ABIN968694

Dieses Anti-Synaptophysin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Synaptophysin in WB, IF und BI. Geeignet für Human, Ratte und Maus. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für Synaptophysin Antikörper (AA 205-306) (ABIN968694)

Target: Synaptophysin (SYP)

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Synaptophysin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Klon: 2-Synaptophysin

Produktdetails anti-Synaptophysin Antikörper

Bindungsspezifität: AA 205-306

Kreuzreaktivität: Human, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Synaptophysin aa. 205-306

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Synaptophysin Antikörper (ABIN968694)

Daly, Sugimori, Moreira, Ziff, Llinás: "Synaptophysin regulates clathrin-independent endocytosis of synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 11, pp. 6120-5, (2000) (PubMed).

Thiele, Hannah, Fahrenholz, Huttner: "Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles." in: Nature cell biology, Vol. 2, Issue 1, pp. 42-9, (2000) (PubMed).

Leube, Kaiser, Seiter, Zimbelmann, Franke, Rehm, Knaus, Prior, Betz, Reinke: "Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA." in: The EMBO journal, Vol. 6, Issue 11, pp. 3261-8, (1988) (PubMed).

Details zu Synaptophysin

Target: Synaptophysin (SYP)

Andere Bezeichnung: Synaptophysin

Hintergrund: Synaptophysin is a major integral membrane glycoprotein that was isolated from the presynaptic vesicles of neurons. The structure of synaptophysin includes four transmembrane domains (TMD), an N-temrinal glycosylation site, nine repeats of basic tetrapeptide motifs (GYGP), multiple collagenase sensitive sites (XGP), and several collagen-type tripeptides (GXX). Synaptophysin mRNA is expressed in nervous tissues, as well as many neuroendocrine tumor cell lines. In PC12 cells and brain synaptic vesicles, synaptophysin binds cholesterol, and cholesterol depletion blocks the biogenesis of synaptic-like microvesicles. In addition, synaptophysin binds dynamin in a Ca2+-dependent manner, and injection of peptides that disrupt this interaction decreases neurotransmitter release during high-frequency stimulation of squid neurons. This decrease in neurotransmitter release results from inhibition of synaptophysin-dependent vesicle retrieval. Thus, synaptophysin may have important roles in the biogenesis and recycling of synaptic vesicles. Synaptophysin is an integral membrane glycoprotein with a calculated molecular weight of 33 kD, but has been reported to be observed at 38-42 kD due to glycosylation. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 38 kDa

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity