AKAP4 Antikörper (AA 555-675)

Produktnummer ABIN968592

Dieses Anti-AKAP4-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von AKAP4 in WB und IF. Geeignet für Maus und Ratte. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für AKAP4 Antikörper (AA 555-675) (ABIN968592)

Target: AKAP4 (A Kinase (PRKA) Anchor Protein 4 (AKAP4))

Reaktivität: Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AKAP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 25-AKAP82

Produktdetails anti-AKAP4 Antikörper

Bindungsspezifität: AA 555-675

Kreuzreaktivität: Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse AKAP82 aa. 555-675

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-AKAP4 Antikörper (ABIN968592)

Edwards, Scott: "A-kinase anchoring proteins: protein kinase A and beyond." in: Current opinion in cell biology, Vol. 12, Issue 2, pp. 217-21, (2000) (PubMed).

Miki, Eddy: "Identification of tethering domains for protein kinase A type Ialpha regulatory subunits on sperm fibrous sheath protein FSC1." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 34384-90, (1999) (PubMed).

Turner, Johnson, Haig-Ladewig, Gerton, Moss: "An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail." in: The Journal of biological chemistry, Vol. 273, Issue 48, pp. 32135-41, (1998) (PubMed).

Fulcher, Mori, Welch, OBrien, Klapper, Eddy: "Characterization of Fsc1 cDNA for a mouse sperm fibrous sheath component." in: Biology of reproduction, Vol. 52, Issue 1, pp. 41-9, (1995) (PubMed).

Details zu AKAP4

Target: AKAP4 (A Kinase (PRKA) Anchor Protein 4 (AKAP4))

Andere Bezeichnung: AKAP82

Hintergrund: The cAMP-dependent Protein Kinase (PKA) is compartmentalized within the cell. To maintain the localization of PKA, the regulatory subunits interact with specific anchoring proteins. Several proteins have been identified as PKA anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). Fifteen of the AKAP proteins contain a consensus binding motif that allows interaction with the type II regulatory subunit (RII) of the PKA holoenzyme. In addition, three other AKAPs (D-AKAP1, D-AKAP2, and fsc1/AKAP82) can associate with the type I regulatory subunit (RI) of the PKA holoenzyme. AKAP82 was isolated as a component of the mouse sperm fibrous sheath. It is a dual specificity AKAP that contains an RII-binding domain (domain A, amino acids 219 to 232) and an RI-binding domain (domain B, amino acids 335-344). In mouse, pro-AKAP82 is synthesized as a 97 kDa precursor that is transported to the flagellum where proteolytic cleavage of the N-terminal 179 amino acids produces AKAP82. Assembly of AKAP82 into the fibrous sheath surrounding the axoneme of the sperm flagellum is thought to tether PKA close to the axoneme where it can regulate flagellar motility.

Molekulargewicht: 82 kDa