FUS Antikörper (AA 2-117)

Produktnummer ABIN968509

Produktdetails anti-FUS Antikörper

Target: FUS (Fused in Sarcoma (FUS))

Bindungsspezifität: AA 2-117

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FUS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TLS aa. 2-117.

Klon: 15-TLS

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-FUS Antikörper (ABIN968509)

Hallier, Lerga, Barnache, Tavitian, Moreau-Gachelin: "The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4838-42, (1998) (PubMed).

Morohoshi, Ootsuka, Arai, Ichikawa, Mitani, Munakata, Ohki: "Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes." in: Gene, Vol. 221, Issue 2, pp. 191-8, (1998) (PubMed).

Powers, Mathur, Raaka, Ron, Samuels: "TLS (translocated-in-liposarcoma) is a high-affinity interactor for steroid, thyroid hormone, and retinoid receptors." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 1, pp. 4-18, (1998) (PubMed).

Yang, Embree, Tsai, Hickstein: "Oncoprotein TLS interacts with serine-arginine proteins involved in RNA splicing." in: The Journal of biological chemistry, Vol. 273, Issue 43, pp. 27761-4, (1998) (PubMed).

Zinszner, Sok, Immanuel, Yin, Ron: "TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling." in: Journal of cell science, Vol. 110 ( Pt 15), pp. 1741-50, (1997) (PubMed).

Details zu FUS

Target: FUS (Fused in Sarcoma (FUS))

Andere Bezeichnung: TLS (FUS Produkte)

Substanzklasse: Viral Protein

Hintergrund: Chromosomal translocations in leukemias and malignancies of non-hematopoietic tissues usually result in the fusion of genes and the production of novel fusion proteins. Translocated in liposarcoma (TLS/FUS) was identified through its fusion to the transcription factor CHOP in human myxoid liposarcoma. In addition, the fusion of TLS to the transcription factor ERG is involved in human acute myeloid leukemia. TLS contains an N-terminal Gln-, Ser-, and Tyr-rich region (QSY) that is thought to be a potent transactivator when fused with transcription factors. In its C-terminus, TLS contains a ribonucleoprotein consensus sequence (RNP-CS) and Arg-Gly-Gly (RGG) repeats that have been implicated in RNA binding. This feature allows it to function in shuttling of RNA from the nucleus. TLS also interacts with Ser-Arg proteins that regulate RNA splicing. Interestingly, TLS also interacts with the DNA binding domains of the estrogen, thyroid hormone, and glucocorticoid receptors. Thus, TLS is a multifunctional protein that is involved in RNA transport, nuclear receptor function, and gene transactivation when fused with transcription factors. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 65 kDa