Ataxin 2 Antikörper (AA 713-904)

Produktnummer ABIN968504

Der Maus Monoklonal Anti-Ataxin 2-Antikörper wurde für WB und IF validiert. Er ist geeignet, Ataxin 2 in Proben von Human und Ratte zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für Ataxin 2 Antikörper (AA 713-904) (ABIN968504)

Target: Ataxin 2 (ATXN2)

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ataxin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 22-Ataxin

Produktdetails anti-Ataxin 2 Antikörper

Bindungsspezifität: AA 713-904

Kreuzreaktivität: Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ataxin-2 aa. 713-904

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Ataxin 2 Antikörper (ABIN968504)

Huynh, Del Bigio, Ho, Pulst: "Expression of ataxin-2 in brains from normal individuals and patients with Alzheimer's disease and spinocerebellar ataxia 2." in: Annals of neurology, Vol. 45, Issue 2, pp. 232-41, (1999) (PubMed).

Nechiporuk, Huynh, Figueroa, Sahba, Nechiporuk, Pulst: "The mouse SCA2 gene: cDNA sequence, alternative splicing and protein expression." in: Human molecular genetics, Vol. 7, Issue 8, pp. 1301-9, (1998) (PubMed).

Pulst, Nechiporuk, Nechiporuk, Gispert, Chen, Lopes-Cendes, Pearlman, Starkman, Orozco-Diaz, Lunkes, DeJong, Rouleau, Auburger, Korenberg, Figueroa, Sahba: "Moderate expansion of a normally biallelic trinucleotide repeat in spinocerebellar ataxia type 2." in: Nature genetics, Vol. 14, Issue 3, pp. 269-76, (1996) (PubMed).

Details zu Ataxin 2

Target: Ataxin 2 (ATXN2)

Andere Bezeichnung: Ataxin-2

Hintergrund: The hereditary ataxias are neurodegenerative disorders characterized by abnormalities of balance due to dysfunction of the cerebellum and cerebellar pathways. Spinocerebellar ataxias (SCAs) represent a heterogeneous group of disorders with a prevalence of about 1 in 10^5. Moderate expansion (36 or 37 units) of CAG repeats on the SCA2 gene leads to a gene product, ataxin-2, that has a long polyglutamine tract. Ataxin-2 is a basic protein with two domains (Sm1 and Sm2) that have been implicated in RNA splicing and protein interaction. Human ataxin-2 has significant homology with mouse ataxin-2 and ataxin-2 related protein. Ataxin-2 is expressed as early as day 8 of mouse embryogenesis and is detected in brain, heart, placenta, liver, skeletal muscle, and pancreas, but not in lung or kidney. Expression increases with age and the protein is localized to the cytoplasm in purkinje cells and other neuronal cell types. Thus, ataxin-2 is thought to be important for RNA splicing or protein complex formation in many normal tissues, while polyglutamine containing ataxin-2 leads to neuropathology.

Molekulargewicht: 150 kDa

Pathways: Ribonucleoprotein Complex Subunit Organization