CIT Antikörper (AA 1420-1612)

Produktnummer ABIN968502

Dieses Anti-CIT-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CIT in WB und IF. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für CIT Antikörper (AA 1420-1612) (ABIN968502)

Target: CIT (Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CIT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 6-CRIK

Produktdetails anti-CIT Antikörper

Bindungsspezifität: AA 1420-1612

Kreuzreaktivität: Human, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CRIK aa. 1420-1612

Isotyp: IgG1 kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN968539, ABIN967389, ABIN968537

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-CIT Antikörper (ABIN968502)

Furuyashiki, Fujisawa, Fujita, Madaule, Uchino, Mishina, Bito, Narumiya: "Citron, a Rho-target, interacts with PSD-95/SAP-90 at glutamatergic synapses in the thalamus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 1, pp. 109-18, (1999) (PubMed).

Zhang, Vazquez, Apperson, Kennedy: "Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 1, pp. 96-108, (1999) (PubMed).

Di Cunto, Calautti, Hsiao, Ong, Topley, Turco, Dotto: "Citron rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein Citron." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29706-11, (1998) (PubMed).

Madaule, Eda, Watanabe, Fujisawa, Matsuoka, Bito, Ishizaki, Narumiya: "Role of citron kinase as a target of the small GTPase Rho in cytokinesis." in: Nature, Vol. 394, Issue 6692, pp. 491-4, (1998) (PubMed).

Details zu CIT

Target: CIT (Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT))

Andere Bezeichnung: CRIK

Hintergrund: Citron was identified by its interactions with activated Rho and Rac. It contains a zinc finger (ZF) region, a pleckstrin homology (PH) domain, a citron/MRCK/Gek (CMG) domain, and a coiled-coil domain with a Rho-Rac binding region (RRB). Citron binds to PSD-95 via its C-terminal tSXV motif and the PDZ domain on PSD-95. This association occurs at post synaptic densities where citron-PSD-95 may provide a specific link between the Rho signaling cascade and the synaptic NMDA receptor complex. Citron Rho-interacting kinase (CRIK) and CRIK short kinase (CRIK-SK) are Ser/Thr kinase isoforms of the myotonic dystrophy kinase family. CRIK contains full-length citron, with an N-terminal kinase domain. CRIK-SK is a splice variant that contains only the kinase domain. CRIK co-localizes with actin in keratinocytes and co-expression of CRIK and Rho increases CRIK kinase activity two-fold. In addition, CRIK mutants cause abnormal contraction during cytokinesis. CRIK is expressed highest in testis, but is also present in brain, spleen, lung, and kidney. Thus, CRIK is a Ser/Thr kinase that functions downstream of Rho during events such as cytokinesis and synaptic transmission.
Synonyms: Serine/Threonine Protein Kinase 21

Molekulargewicht: 240 kDa

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion