BCAT1 Antikörper (AA 213-335)

Produktnummer ABIN968446

Dieses Maus Monoklonal-Antikörper erkennt spezifisch BCAT1 in WB und IF. Er zeigt eine Reaktivität gegenüber Human und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für BCAT1 Antikörper (AA 213-335) (ABIN968446)

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BCAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 51-ECA39

Produktdetails anti-BCAT1 Antikörper

Bindungsspezifität: AA 213-335

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ECA39 aa. 213-335

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968535

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-BCAT1 Antikörper (ABIN968446)

Ben-Yosef, Yanuka, Halle, Benvenisty: "Involvement of Myc targets in c-myc and N-myc induced human tumors." in: Oncogene, Vol. 17, Issue 2, pp. 165-71, (1998) (PubMed).

Ben-Yosef, Yanuka, Benvenisty: "ECA39 is regulated by c-Myc in human and by a Jun/Fos homolog, Gcn4, in yeast." in: Oncogene, Vol. 13, Issue 9, pp. 1859-66, (1997) (PubMed).

Eden, Simchen, Benvenisty: "Two yeast homologs of ECA39, a target for c-Myc regulation, code for cytosolic and mitochondrial branched-chain amino acid aminotransferases." in: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20242-5, (1996) (PubMed).

Schuldiner, Eden, Ben-Yosef, Yanuka, Simchen, Benvenisty: "ECA39, a conserved gene regulated by c-Myc in mice, is involved in G1/S cell cycle regulation in yeast." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 14, pp. 7143-8, (1996) (PubMed).

Details zu BCAT1

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Andere Bezeichnung: ECA39

Hintergrund: The c-myc oncogene is important in proliferation, differentiation, apoptosis, and malignant transformation. Products of myc oncogenes are transcription factors that bind to the DNA sequence, CACGTG, and regulate the expression of multiple target genes. Several myc target genes include ECA39, p53, ornithine decarboxylase (ODC), alpha-prothymosin, and Cdc25A. There is a high degree of identity among the mouse, human, and yeast ECA39 proteins. The myc recognition binding site of ECA39 is located 3' to the start site of transcription in the mouse and human genes, but this element is absent in the nematode and yeast. Additionally, the tissue specific expression of ECA39 during embryogenesis is similar between human and mouse. Disruption of the ECA39 gene in yeast results in an increased growth rate in comparison to wild type, such that G1 is shorter. Furthermore, ECA39 is expressed in c-myc induced tumors and displays significant homology to the prokaryotic branched-chain amino acid aminotransferase (BCAT). Thus, ECA39 may be involved in the regulation of the cell cycle, possibly at the G1 to S phase transition.

Molekulargewicht: 43 kDa