VTI1A Antikörper (AA 114-217)

Produktnummer ABIN968427

Dieses Anti-VTI1A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von VTI1A in WB und IF. Geeignet für Human, Maus, Ratte und Hund. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für VTI1A Antikörper (AA 114-217) (ABIN968427)

Target: VTI1A (Vesicle Transport through Interaction with t-SNAREs 1A (VTI1A))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VTI1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 45-Vti1a

Produktdetails anti-VTI1A Antikörper

Bindungsspezifität: AA 114-217

Kreuzreaktivität: Ratte (Rattus), Human, Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Vti1a aa.114-217

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968545

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-VTI1A Antikörper (ABIN968427)

Chiu, Novikov, Mukherjee, Shields: "A caspase cleavage fragment of p115 induces fragmentation of the Golgi apparatus and apoptosis." in: The Journal of cell biology, Vol. 159, Issue 4, pp. 637-48, (2002) (PubMed).

Mallard, Tang, Galli, Tenza, Saint-Pol, Yue, Antony, Hong, Goud, Johannes: "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform." in: The Journal of cell biology, Vol. 156, Issue 4, pp. 653-64, (2002) (PubMed).

Shorter, Beard, Seemann, Dirac-Svejstrup, Warren: "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115." in: The Journal of cell biology, Vol. 157, Issue 1, pp. 45-62, (2002) (PubMed).

Advani, Bae, Bock, Chao, Doung, Prekeris, Yoo, Scheller: "Seven novel mammalian SNARE proteins localize to distinct membrane compartments." in: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10317-24, (1998) (PubMed).

Xu, Wong, Tang, Subramaniam, Zhang, Hong: "A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (Vti1-rp2) implicated in protein trafficking in the secretory pathway." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21783-9, (1998) (PubMed).

Details zu VTI1A

Target: VTI1A (Vesicle Transport through Interaction with t-SNAREs 1A (VTI1A))

Andere Bezeichnung: Vti1a

Hintergrund: Eukaryotic protein trafficking involves the packaging of molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs) and target proteins (t-SNAREs) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. VTI1a and VTI1b are putative mammalian SNARE proteins identified by sequence comparison with yeast SNAREs. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. Vti1a, a possible t-SNARE, contains a C-terminal hydrophobic domain and several regions that may form coiled-coil structures. It exists in distinct syntaxin 5- and syntaxin 6-containing SNARE complexes within the Golgi apparatus. Inhibition of Vti1a blocks transport of G proteins to the cell surface and results in their accumulation within the Golgi. Thus, Vti1a functions in protein transport within the secretory pathway.

Molekulargewicht: 29 kDa