KIF11 Antikörper (AA 324-532)

Produktnummer ABIN968407

Dieses Maus Monoklonal-Antikörper erkennt spezifisch KIF11 in WB und IF. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für KIF11 Antikörper (AA 324-532) (ABIN968407)

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KIF11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 20-EG5

Produktdetails anti-KIF11 Antikörper

Bindungsspezifität: AA 324-532

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Eg5 aa. 324-532

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-KIF11 Antikörper (ABIN968407)

Ferhat, Cook, Chauviere, Harper, Kress, Lyons, Baas: "Expression of the mitotic motor protein Eg5 in postmitotic neurons: implications for neuronal development." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 19, pp. 7822-35, (1998) (PubMed).

Blangy, Lane, dHérin, Harper, Kress, Nigg: "Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo." in: Cell, Vol. 83, Issue 7, pp. 1159-69, (1996) (PubMed).

Details zu KIF11

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Andere Bezeichnung: Eg5

Hintergrund: The mitotic spindle apparatus equally distributes duplicated chromosomes to daughter cells. This process is mediated by the dynamic instability of the spindle microtubules and the forces generated by the action of cytoplasmic dynein and kinesin-related motor proteins (KRPs), such as CHO1/MKLP1. These motors hydrolyze ATP as they move progressively along the microtubules. They organize the microtubules into bipolar spindles and may also serve as bridges between microtubules and chromosome kinetochores or centrosomes. Eg5, identified in Xenopus laevis, is a plus-end directed KRP, associated with the mitotic spindle. Vertebrate Eg5 is a member of the KRP subfamily bimC. These family members share a high degree of sequence homology (>80% within the N-terminal motor domain) and may execute similiar functions. During mitosis, Eg5 is specifically phosphorylated at Thr-297, an evolutionarily conserved cdc2 phosphorylation site, by p34[cdc2]/cyclin B. Inhibition of phosphorylation blocks the interaction of Eg5 with centrosomes. Thus, Eg5 is a mitotic motor protein that regulates spindle formation in a phosphorylation-dependent manner. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 120 kDa