SATB1 Antikörper (AA 550-667)

Produktnummer ABIN968403

Dieses Anti-SATB1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SATB1 in WB und IF. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 7+ Publikationen zitiert.

Kurzübersicht für SATB1 Antikörper (AA 550-667) (ABIN968403)

Target: SATB1 (SATB Homeobox 1 (SATB1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SATB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 14-SATB1

Produktdetails anti-SATB1 Antikörper

Bindungsspezifität: AA 550-667

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SATB1 aa. 550-667

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-SATB1 Antikörper (ABIN968403)

Skowronska-Krawczyk, Ma, Schwartz, Scully, Li, Liu, Taylor, Tollkuhn, Ohgi, Notani, Kohwi, Kohwi-Shigematsu, Rosenfeld: "Required enhancer-matrin-3 network interactions for a homeodomain transcription program." in: Nature, Vol. 514, Issue 7521, pp. 257-61, (2014) (PubMed).

Beyer, Thabet, Müller, Sadlon, Classen, Lahl, Basu, Zhou, Bailey-Bucktrout, Krebs, Schönfeld, Böttcher, Golovina, Mayer, Hofmann, Sommer, Debey-Pascher, Endl, Limmer, Hippen, Blazar, Balderas, Quast et al.: "Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation. ..." in: Nature immunology, Vol. 12, Issue 9, pp. 898-907, (2011) (PubMed).

Alvarez, Yasui, Niida, Joh, Loh, Kohwi-Shigematsu: "The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development." in: Genes & development, Vol. 14, Issue 5, pp. 521-35, (2000) (PubMed).

Cai, Kohwi-Shigematsu: "Intranuclear relocalization of matrix binding sites during T cell activation detected by amplified fluorescence in situ hybridization." in: Methods (San Diego, Calif.), Vol. 19, Issue 3, pp. 394-402, (2000) (PubMed).

de Belle, Cai, Kohwi-Shigematsu: "The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops." in: The Journal of cell biology, Vol. 141, Issue 2, pp. 335-48, (1998) (PubMed).

Liu, Bramblett, Zhu, Lozano, Kobayashi, Ross, Dudley: "The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expression." in: Molecular and cellular biology, Vol. 17, Issue 9, pp. 5275-87, (1997) (PubMed).

Dickinson, Joh, Kohwi, Kohwi-Shigematsu: "A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition." in: Cell, Vol. 70, Issue 4, pp. 631-45, (1992) (PubMed).

Details zu SATB1

Target: SATB1 (SATB Homeobox 1 (SATB1))

Andere Bezeichnung: SATB1

Hintergrund: Packaging of fully functional eukaryotic chromosomal DNA within the nucleus requires a highly ordered organizational process. Central to this is the formation of nucleosomes, which consist of octameric histone cores with defined segments of chromatin wound around them, and the interaction of nucleosomes with nuclear matrix components. Such specific genomic DNA segments that interact with the nuclear matrix are called scaffold or matrix attachment regions (SARs or MARs). SATB1 (Special AT-rich sequence-Binding protein 1), expressed predominantly in the thymus, is a homeodomain MAR binding protein. It recognizes ATC sequences that consist of stretches of A's, T's, and C's on one DNA strand. These sequences tend to unwind by extensive base-unpairing in response to negative superhelical strain. SATB1 exclusively binds double stranded DNA in the minor groove with minimal base contact and it has a low affinity for supercoiled DNA. In addition, SATB1 is thought to act as a transcriptional repressor. SATB1 is a MAR binding protein that binds to the base of chromatin loops and may be essential to T cell-specific gene regulation. This antibody is routinely tested by western blot analysis.
Synonyms: Special AT-rich sequence-Binding protein-1

Molekulargewicht: 106 kDa

Pathways: Caspase Kaskade in der Apoptose, Activated T Cell Proliferation