MAP4 Antikörper (AA 583-702)

Produktnummer ABIN968326

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MAP4 in WB und IF. Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für MAP4 Antikörper (AA 583-702) (ABIN968326)

Target: MAP4 (Microtubule-Associated Protein 4 (MAP4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 18-MAP4

Produktdetails anti-MAP4 Antikörper

Bindungsspezifität: AA 583-702

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human MAP4 aa. 583-702

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-MAP4 Antikörper (ABIN968326)

Drewes, Ebneth, Preuss, Mandelkow, Mandelkow: "MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption." in: Cell, Vol. 89, Issue 2, pp. 297-308, (1997) (PubMed).

Ulitzur, Humbert, Pfeffer: "Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5084-9, (1997) (PubMed).

Chapin, Lue, Yu, Bulinski: "Differential expression of alternatively spliced forms of MAP4: a repertoire of structurally different microtubule-binding domains." in: Biochemistry, Vol. 34, Issue 7, pp. 2289-301, (1995) (PubMed).

Details zu MAP4

Target: MAP4 (Microtubule-Associated Protein 4 (MAP4))

Andere Bezeichnung: MAP4

Hintergrund: The microtubule (MT) cytoskeleton functions in cytoplasmic organization, cellular movement, determination of cell polarity, intracellular transport, and chromosome segregation. Dynamic instability, continuous cycles of MT assembly (stabilization) and disassembly, mediates MT participation in these events. The function of MTs, particularly stabilization, is regulated by MT-associated proteins (MAPs). A subfamily of MAPs, called AP-MAPs (assembly promoting MAPs), includes tau, MAP2, and MAP4. These proteins are classified as type II MAPs and contain a C-terminal MT binding domain with 3 to 5 imperfect repeats of an 18 amino acid motif. While tau and MAP2 are specifically expressed in neuronal cells, MAP4 is the major MAP of nonneuronal mammalian cells. MAP activity and interaction with MTs are regulated by MARK (MAP/MT affinity-regulating kinase) and mapmodulin. MARK-mediated phosphorylation of MAPs on their homologous KXGS motifs results in detachment of MAPs from MTs and MT disruption. In addition, mapmodulin tightly interacts with the MT-binding domains of MAPs and hinders MAP binding to MTs. Thus, MAP4 is a highly regulated type II MAP that controls MT assembly and disassembly.

Molekulargewicht: 200-220 kDa

Pathways: p53 Signalweg, Microtubule Dynamics