SRP54 Antikörper (AA 262-476)

Produktnummer ABIN968262

Dieses Anti-SRP54-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SRP54 in WB und IF. Geeignet für Human, Maus, Ratte, Hund und Frosch. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für SRP54 Antikörper (AA 262-476) (ABIN968262)

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Reaktivität: Human, Maus, Ratte, Hund, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SRP54 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 30-SRP54

Produktdetails anti-SRP54 Antikörper

Bindungsspezifität: AA 262-476

Kreuzreaktivität: Hund, Ratte (Rattus), Maus, Frosch

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SRP54 aa. 262-476

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-SRP54 Antikörper (ABIN968262)

Rapiejko, Gilmore: "Empty site forms of the SRP54 and SR alpha GTPases mediate targeting of ribosome-nascent chain complexes to the endoplasmic reticulum." in: Cell, Vol. 89, Issue 5, pp. 703-13, (1997) (PubMed).

Traianedes, Findlay, Martin, Gillespie: "Modulation of the signal recognition particle 54-kDa subunit (SRP54) in rat preosteoblasts by the extracellular matrix." in: The Journal of biological chemistry, Vol. 270, Issue 36, pp. 20891-4, (1995) (PubMed).

Details zu SRP54

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Andere Bezeichnung: SRP54

Hintergrund: Ribosomes that exist freely in the cytosol or those attached to the ER are intrinsically the same in their translational properties. ER-bound ribosomes are responsible for the production of secretory proteins and integral ER, Golgi, lysosomal, and plasma membrane spanning proteins. Such proteins contain signal sequences that direct their synthesis to the ER membrane. As the nascent polypeptide emerges from the ribosome, a signal recognition particle (SRP) binds to the signal sequence and serves to couple the ribosome to the protein-translocating machinery in the ER membrane. Although the SRP is a 325 kDa ribonucleoprotein, its 54 kDa subunit (SRP54) mediates interaction with, and targeting of, the nascent protein to the ER. Via its C-terminal M-domain, SRP54 associates with the nascent protein and inhibits its elongation. This complex binds to the SRP receptor on the ER, the ribosome is delivered to the translocation machinery, SRP is released, and elongation resumes. Targeting and insertion are tightly coupled to a GTPase cycle that involves SRP54 and SRP receptor. Although the mechanisms are unclear, release of SRP from the ER-bound complex requires GTP hydrolysis.

Molekulargewicht: 54 kDa

Pathways: SARS-CoV-2 Protein Interaktom