TFCP2 Antikörper (AA 205-414)

Produktnummer ABIN968193

Dieses Anti-TFCP2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TFCP2 in WB und IF. Geeignet für Human, Maus, Ratte und Hund. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für TFCP2 Antikörper (AA 205-414) (ABIN968193)

Target: TFCP2 (Transcription Factor CP2 (TFCP2))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TFCP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 14-LSF

Produktdetails anti-TFCP2 Antikörper

Bindungsspezifität: AA 205-414

Kreuzreaktivität: Maus, Ratte (Rattus), Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human LSF aa. 205-414

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968551, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-TFCP2 Antikörper (ABIN968193)

Volker, Rameh, Zhu, DeCaprio, Hansen: "Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity." in: Genes & development, Vol. 11, Issue 11, pp. 1435-46, (1997) (PubMed).

Shirra, Zhu, Huang, Pallas, Hansen: "One exon of the human LSF gene includes conserved regions involved in novel DNA-binding and dimerization motifs." in: Molecular and cellular biology, Vol. 14, Issue 8, pp. 5076-87, (1994) (PubMed).

Details zu TFCP2

Target: TFCP2 (Transcription Factor CP2 (TFCP2))

Andere Bezeichnung: LSF

Hintergrund: LSF (alphaCP2 or LBP-1c) is a transcription factor originally identified by its binding to, and induction of, the SV40 late promoter. LSF displays some similarity to the Elf-1/NTF transcription factors of Drosophila. It is primarily found in its dimeric form in solution, however it is the tetrameric form that recognizes a center-to-center 10 bp repeat. LSF-1D (LBP-1d) is the product of an alternative splicing event, resulting in a deletion of a 51 amino acid segment that corresponds with the DNA-binding region of LSF. LSF-1D is found in the cytoplasm, not in the nucleus, and can interact with LSF to form a heterotetramer that can interfere with LSF DNA binding. In mitogen-stimulated human peripheral T cells, LSF is phosphorylated on amino acid 291, which increases its DNA binding activity. Thus, LSF-mediated gene transcription is regulated by its phosphorylation and/or its interaction with LSF-1D during cell growth. This antibody is routinely tested by western blot analysis.
Synonyms: alphaCP2, LBP-1c

Molekulargewicht: 63 kDa