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Afadin Antikörper (AA 1091-1233)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Afadin in WB, IHC, IP und IF. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus und wurde in 5+ Publikationen erwähnt.
Produktnummer ABIN968143

Kurzübersicht für Afadin Antikörper (AA 1091-1233) (ABIN968143)

Target

Alle Afadin (MLLT4) Antikörper anzeigen
Afadin (MLLT4)

Reaktivität

  • 25
  • 14
  • 14
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte, Maus

Wirt

  • 23
  • 2
  • 1
Maus

Klonalität

  • 25
  • 1
Monoklonal

Konjugat

  • 15
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Afadin Antikörper ist unkonjugiert

Applikation

  • 14
  • 7
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Klon

35-AF6
  • Bindungsspezifität

    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1091-1233

    Kreuzreaktivität

    Ratte (Rattus), Maus

    Produktmerkmale

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Please refer to us for technical protocols.

    Aufreinigung

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Immunogen

    Human AF6 aa. 1091-1233

    Isotyp

    IgG2a
  • Kommentare

    Related Products: ABIN968545, ABIN967389

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    250 μg/mL

    Buffer

    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store undiluted at -20° C.
  • Cordenonsi, DAtri, Hammar, Parry, Kendrick-Jones, Shore, Citi: "Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin." in: The Journal of cell biology, Vol. 147, Issue 7, pp. 1569-82, (2000) (PubMed).

    Ebnet, Schulz, Meyer Zu Brickwedde, Pendl, Vestweber: "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27979-88, (2000) (PubMed).

    Buchert, Schneider, Meskenaite, Adams, Canaani, Baechi, Moelling, Hovens: "The junction-associated protein AF-6 interacts and clusters with specific Eph receptor tyrosine kinases at specialized sites of cell-cell contact in the brain." in: The Journal of cell biology, Vol. 144, Issue 2, pp. 361-71, (1999) (PubMed).

    Kuriyama, Harada, Kuroda, Yamamoto, Nakafuku, Iwamatsu, Yamamoto, Prasad, Croce, Canaani, Kaibuchi: "Identification of AF-6 and canoe as putative targets for Ras." in: The Journal of biological chemistry, Vol. 271, Issue 2, pp. 607-10, (1996) (PubMed).

    Prasad, Gu, Alder, Nakamura, Canaani, Saito, Huebner, Gale, Nowell, Kuriyama: "Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in acute myeloid leukemias with the t(6;11) chromosome translocation." in: Cancer research, Vol. 53, Issue 23, pp. 5624-8, (1994) (PubMed).

  • Target

    Afadin (MLLT4)

    Andere Bezeichnung

    AF6

    Hintergrund

    AF6 (ALL-1 Fusion partner in chromosome 6) was discovered as the fusion partner of ALL-1. These genes undergo translocation and fusion in acute myeloid leukemias. AF6, or p180, was also identified as a Ras-interacting protein isolated from bovine brain. AF6 binds to the GTP-Ras but not to the inactive GDP, or ineffective forms of Ras. The interaction between AF6 and Ras may regulate the Ras signaling pathway since this association was prevented by the binding of c-Raf-1 to Ras. The Ras-binding site is located within amino acids 36-206 of AF6. AF6 is 1612 amino acids in length with homology to the DLG family of proteins located in cell-cell junctions, suggesting AF6 might be important in cell-to-cell communicative events. Furthermore, AF6 has the GLGF motif characteristic of DLG proteins that include Drosophilia's Canoe, implicated in the developmental pathway triggered by Notch activation. Like AF6, Canoe was also identified as a Ras-binding protein. The amino acid sequence and biochemical property similarities between Canoe and AF6 indicate that the latter may be important in developmental processes regulated by Ras.
    Synonyms: ALL-1 Fusion partner in chromosome 6

    Molekulargewicht

    182 kDa

    Pathways

    Cell-Cell Junction Organization
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