Afadin Antikörper (AA 1091-1233)

Produktnummer ABIN968143

Produktdetails anti-Afadin Antikörper

Target: Afadin (MLLT4)

Bindungsspezifität: AA 1091-1233

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Afadin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Kreuzreaktivität: Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human AF6 aa. 1091-1233

Klon: 35-AF6

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Afadin Antikörper (ABIN968143)

Cordenonsi, DAtri, Hammar, Parry, Kendrick-Jones, Shore, Citi: "Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin." in: The Journal of cell biology, Vol. 147, Issue 7, pp. 1569-82, (2000) (PubMed).

Ebnet, Schulz, Meyer Zu Brickwedde, Pendl, Vestweber: "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27979-88, (2000) (PubMed).

Buchert, Schneider, Meskenaite, Adams, Canaani, Baechi, Moelling, Hovens: "The junction-associated protein AF-6 interacts and clusters with specific Eph receptor tyrosine kinases at specialized sites of cell-cell contact in the brain." in: The Journal of cell biology, Vol. 144, Issue 2, pp. 361-71, (1999) (PubMed).

Kuriyama, Harada, Kuroda, Yamamoto, Nakafuku, Iwamatsu, Yamamoto, Prasad, Croce, Canaani, Kaibuchi: "Identification of AF-6 and canoe as putative targets for Ras." in: The Journal of biological chemistry, Vol. 271, Issue 2, pp. 607-10, (1996) (PubMed).

Prasad, Gu, Alder, Nakamura, Canaani, Saito, Huebner, Gale, Nowell, Kuriyama: "Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in acute myeloid leukemias with the t(6;11) chromosome translocation." in: Cancer research, Vol. 53, Issue 23, pp. 5624-8, (1994) (PubMed).

Details zu Afadin

Target: Afadin (MLLT4)

Andere Bezeichnung: AF6 (MLLT4 Produkte)

Hintergrund: AF6 (ALL-1 Fusion partner in chromosome 6) was discovered as the fusion partner of ALL-1. These genes undergo translocation and fusion in acute myeloid leukemias. AF6, or p180, was also identified as a Ras-interacting protein isolated from bovine brain. AF6 binds to the GTP-Ras but not to the inactive GDP, or ineffective forms of Ras. The interaction between AF6 and Ras may regulate the Ras signaling pathway since this association was prevented by the binding of c-Raf-1 to Ras. The Ras-binding site is located within amino acids 36-206 of AF6. AF6 is 1612 amino acids in length with homology to the DLG family of proteins located in cell-cell junctions, suggesting AF6 might be important in cell-to-cell communicative events. Furthermore, AF6 has the GLGF motif characteristic of DLG proteins that include Drosophilia's Canoe, implicated in the developmental pathway triggered by Notch activation. Like AF6, Canoe was also identified as a Ras-binding protein. The amino acid sequence and biochemical property similarities between Canoe and AF6 indicate that the latter may be important in developmental processes regulated by Ras.
Synonyms: ALL-1 Fusion partner in chromosome 6

Molekulargewicht: 182 kDa

Pathways: Cell-Cell Junction Organization