RAB5 Antikörper (AA 1-215)

Produktnummer ABIN968139

Dieses Anti-RAB5-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von RAB5 in WB, IHC und IF. Geeignet für Human und Hund. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für RAB5 Antikörper (AA 1-215) (ABIN968139)

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAB5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: 1-Rab5

Produktdetails anti-RAB5 Antikörper

Bindungsspezifität: AA 1-215

Kreuzreaktivität: Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rab5 aa. 1-215

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-RAB5 Antikörper (ABIN968139)

Stenmark, Vitale, Ullrich, Zerial: "Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion." in: Cell, Vol. 83, Issue 3, pp. 423-32, (1996) (PubMed).

Ullrich, Horiuchi, Bucci, Zerial: "Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange." in: Nature, Vol. 368, Issue 6467, pp. 157-60, (1994) (PubMed).

Li, Stahl: "Structure-function relationship of the small GTPase rab5." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 24475-80, (1993) (PubMed).

Sanford, Pan, Wessling-Resnick: "Prenylation of Rab5 is dependent on guanine nucleotide binding." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 23773-6, (1993) (PubMed).

Details zu RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Andere Bezeichnung: Rab5

Hintergrund: Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post-translationally modified by geranylgeranylation which is critical for its membrane targeting. Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 regulates early endosome fusion. The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and membranes. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 may insert into membranes by a multistep process in a which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 25 kDa

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity