Dihydrofolate Reductase Antikörper (AA 1-186)

Produktnummer ABIN968119

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Dihydrofolate Reductase in WB, IHC, IF und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Hund und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für Dihydrofolate Reductase Antikörper (AA 1-186) (ABIN968119)

Target: Dihydrofolate Reductase (DHFR)

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Dihydrofolate Reductase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 49-DHFR

Produktdetails anti-Dihydrofolate Reductase Antikörper

Bindungsspezifität: AA 1-186

Kreuzreaktivität: Maus, Human, Hund, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Cow DHFR aa. 1-186

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Dihydrofolate Reductase Antikörper (ABIN968119)

Eymin, Gazzeri, Brambilla, Brambilla: "Distinct pattern of E2F1 expression in human lung tumours: E2F1 is upregulated in small cell lung carcinoma." in: Oncogene, Vol. 20, Issue 14, pp. 1678-87, (2001) (PubMed).

de Wind, Dekker, Claij, Jansen, van Klink, Radman, Riggins, van der Valk, vant Wout, te Riele: "HNPCC-like cancer predisposition in mice through simultaneous loss of Msh3 and Msh6 mismatch-repair protein functions." in: Nature genetics, Vol. 23, Issue 3, pp. 359-62, (1999) (PubMed).

Tang, Pao, Zhang: "Repair of benzo(a)pyrene diol epoxide- and UV-induced DNA damage in dihydrofolate reductase and adenine phosphoribosyltransferase genes of CHO cells." in: The Journal of biological chemistry, Vol. 269, Issue 17, pp. 12749-54, (1994) (PubMed).

Israel, Kaufman: "Dexamethasone negatively regulates the activity of a chimeric dihydrofolate reductase/glucocorticoid receptor protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 4290-4, (1993) (PubMed).

Masters, Attardi: "The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase." in: Gene, Vol. 21, Issue 1-2, pp. 59-63, (1983) (PubMed).

Details zu Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Andere Bezeichnung: DHFR

Hintergrund: Dihydrofolate reductase (DHFR) regenerates tetrahydrofolate from dihydrofolate in the presence of NADPH. Tetrahydrofolate is the coenzyme for thymidilate synthetase in the biosynthesis of thymidine and is also critical for the synthesis of amino acids and purines. DHFR is a protein of 186 amino acids that is highly conserved among different organisms. DHFR levels change during the cell cycle, with the highest content during the G1/S transition. Expression of DHFR tightly correlates with the turnover rate of its mRNA. Because dividing cells require a continuous supply of thymidine, DHFR has been a target for anticancer drugs. The folic acid antagonists aminopterin and amethopterin (methotrexate), widely used in cancer treatments, inhibit DHFR. However, over long treatments, treated cells often amplify the DHFR gene, producing drug-resistant cells.

Molekulargewicht: 21 kDa

Pathways: Mitotic G1-G1/S Phases